Procleix Ultrio transcription-mediated amplification vs. serological blood screening in south-western Greece

Transfusion Medicine
A SgourouA Mouzaki

Abstract

We present here our overall experience after 27 months of performance of the Procleix Ultrio [HIV-1, hepatitis C virus (HCV), hepatitis B virus (HBV)] transcription-mediated amplification (TMA) assay. The aim of this report is to assess the impact of nucleic acid testing (NAT) implementation in blood screening of south-western Greek blood donors. We processed 38,264 units of blood as neat samples with the Procleix Ultrio TMA assay (Chiron/GenProbe, Emeryville/San Diego, CA, USA) between 1 January 2005 and 31 March 2007. NAT results were compared to those obtained from routine serology tests and quantitative polymerase chain reaction (PCR) assays. Overall, 52 units of blood tested positive for HBV (1.4 per thousand), 8 for HCV (0.2 per thousand) and none for HIV or multiple infections. The yield of TMA was 0.183 per thousand for HBV (7/38 264) and 0 for HCV. The TMA HBV-positive donations were tested for HBV DNA by a quantitative PCR assay and were found negative (below the detection limit of the method, 200 copies/mL). Follow-up testing showed that the TMA HBV-positive donations were positive for anti-hepatitis B core antigen immunoglobulin G antibodies. Implementation of the TMA assay in the individual donation configuration i...Continue Reading

References

Dec 22, 1999·Journal of Viral Hepatitis·M KoulentakiE A Kouroumalis
Apr 13, 2000·Clinical and Laboratory Haematology·J P Allain
Sep 12, 2001·Transfusion·S H Kleinman, M P Busch
Sep 11, 2002·Transfusion Medicine·S L Stramer
Dec 24, 2005·Transfusion·Michael P BuschUNKNOWN National Heart, Lung, Blood Institute Nucleic Acid Test Study Group*
Jul 13, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Steven H Kleinman, Michael P Busch
Nov 16, 2006·Vox Sanguinis·H W Reesink, J-P Allain
Nov 30, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ewa BrojerUNKNOWN Polish Blood Transfusion Service Viral Study Group

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