Procolipase gene expression in the rat brain: source of endogenous enterostatin production in the brain

Brain Research
David A YorkMiejung Park

Abstract

Enterostatin is a pentapeptide released from its precursor protein procolipase, which is synthesized in the exocrine pancreas and gastric mucosa. As central injection of enterostatin has potent effects on feeding, we hypothesized that the procolipase may also be expressed in the brain. We confirmed the presence of preprocolipase gene expression in amygdala by reverse transcription-polymerase chain reaction and Northern blot analysis and of protein expression by Western blots. Immunohistochemical analysis using antibodies for procolipase and enterostatin identified their immunoreactivity (IR) in rat brain. Procolipase IR was present in the cytoplasm of paraventricular, amygdala, and the dorsal thalamus nucleus. Enterostatin IR was evident in the fibers of the dorsal thalamus and arcuate nucleus. In vivo injection of enterostatin antibody into rat amygdala increased food intake. These data suggest that procolipase and enterostatin are synthesized within specific regions of the brain that function in the regulation of food intake centrally.

References

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Dec 2, 2004·Peptides·Miejung ParkDavid A York

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Citations

Jan 4, 2007·The Journal of Clinical Investigation·David E Cummings, Joost Overduin
May 27, 2010·Nutrition Reviews·Jichun YangBryan A Wolf
Aug 1, 2015·International Journal of Obesity : Journal of the International Association for the Study of Obesity·C J RebelloF L Greenway
Oct 16, 2007·Brain Research·Catarina RippeAndreas Lindqvist
Dec 19, 2006·Peptides·Kousaku OhinataMasaaki Yoshikawa
Nov 28, 2012·Neuroscience Letters·H Jonaidi, R Rasooli
Nov 20, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Stéphane BoghossianDavid A York

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