PMID: 41715Oct 15, 1979

Procollagen processing. Limited proteolysis of COOH-terminal extension peptides by a cathepsin-like protease secreted by tendon fibroblasts

European Journal of Biochemistry
J M DavidsonP Bornstein

Abstract

An enzymatic activity, capable of removing the COOH-terminal extensions of type I chick procollagen, has been demonstrated in embryonic chick tendons and in cultured tendon fibroblasts utilizing two new methods of analysis. The protease was purified by a combination of ultrafiltration concanavalin A affinity chromatography and gel filtration. The isolated protein has an apparent Mr of 43,000 by gel filtration and sodium dodecyl sulfate gel electrophoresis. The enzyme shows a major pH optimum at 4.2 and is susceptible to inhibitors such as pepstatin and leupeptin; it therefore seems related to the cathepsins. The possibility that this enzyme plays a role in the limited proteolytic processing of procollagen is discussed.

References

Jan 1, 1978·Annual Review of Biochemistry·J H Fessler, L I Fessler
Apr 1, 1978·FEBS Letters·P G Scott, C H Pearson
Jan 1, 1975·Annual Review of Biochemistry·E F NeufeldL J Shapiro
Jun 1, 1976·Proceedings of the Society for Experimental Biology and Medicine·M B TaubmanB Goldberg
Jul 12, 1976·Biochemical and Biophysical Research Communications·J Uitto, J R Lichtenstein
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·L I FesslerJ H Fessler
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·H P HoffmannD J Prockop
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Apr 1, 1976·Archives of Biochemistry and Biophysics·M B Taubman, B Goldberg
Jan 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·L D KohnC M Lapière
Aug 30, 1972·Nature: New Biology·H P Ehrlich, P Bornstein
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·B Goldberg, C J Sherr
Aug 21, 1973·Biochemical and Biophysical Research Communications·S SakamotoM J Glimcher
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J M Monson, P Borstein
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·G Bellamy, P Bornstein
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Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·C M LapièreL D Kohn

Related Concepts

Cathepsins
Chick Embryo
Fibroblasts
Hydrogen-Ion Concentration
Peptide Hydrolases
Protocollagen
Protease Inhibitors
Tendons, Para-Articular

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