Procyanidin B2 improves endothelial progenitor cell function and promotes wound healing in diabetic mice via activating Nrf2.

Journal of Cellular and Molecular Medicine
Jiawei FanXiaozhen Dai

Abstract

One of the major reasons for the delayed wound healing in diabetes is the dysfunction of endothelial progenitor cells (EPCs) induced by hyperglycaemia. Improvement of EPC function may be a potential strategy for accelerating wound healing in diabetes. Procyanidin B2 (PCB2) is one of the major components of procyanidins, which exhibits a variety of potent pharmacological activities. However, the effects of PCB2 on EPC function and diabetic wound repair remain elusive. We evaluated the protective effects of PCB2 in EPCs with high glucose (HG) treatment and in a diabetic wound healing model. EPCs derived from human umbilical cord blood were treated with HG. The results showed that PCB2 significantly preserved the angiogenic function, survival and migration abilities of EPCs with HG treatment, and attenuated HG-induced oxidative stress of EPCs by scavenging excessive reactive oxygen species (ROS). A mechanistic study found the protective role of PCB2 is dependent on activating nuclear factor erythroid 2-related factor 2 (Nrf2). PCB2 increased the expression of Nrf2 and its downstream antioxidant genes to attenuate the oxidative stress induced by HG in EPCs, which were abolished by knockdown of Nrf2 expression. An in vivo study show...Continue Reading

References

Aug 24, 2004·Circulation Research·Carmen Urbich, Stefanie Dimmeler
Nov 18, 2005·Lancet·Vincent Falanga
Dec 17, 2005·Antioxidants & Redox Signaling·Matthew J CallaghanGeoffrey C Gurtner
Mar 15, 2008·The Journal of Clinical Investigation·Debanjan ChakrobortySujit Basu
Nov 22, 2008·Circulation Journal : Official Journal of the Japanese Circulation Society·Mako OhshimaKimikazu Hamano
Nov 26, 2009·The Journal of International Medical Research·Tomaz VelnarV Smrkolj
Jul 5, 2012·Cold Spring Harbor Perspectives in Medicine·Mervin C Yoder
Dec 3, 2014·Biochemical Pharmacology·Haixia YangNanping Wang
Feb 11, 2015·Reviews in Endocrine & Metabolic Disorders·Rui Cheng, Jian-xing Ma
Jun 30, 2015·Free Radical Biology & Medicine·Shohei Murakami, Hozumi Motohashi
Jun 15, 2017·The New England Journal of Medicine·David G ArmstrongSicco A Bus
Jul 6, 2017·Molecular Medicine Reports·Mei YinHaiqing Gao
Oct 14, 2017·Antioxidants·Taylor C SutcliffeDaniel A Linseman
May 8, 2018·Current Stem Cell Research & Therapy·Divya RanaSaurabh Sharma
Nov 20, 2018·Translational Research : the Journal of Laboratory and Clinical Medicine·Alexander J WhittamGeoffrey C Gurtner
Feb 23, 2015·Regenerative Therapy·Dewi SukmawatiRica Tanaka
Aug 24, 2019·Frontiers in Immunology·Ying TianNanping Wang
Oct 12, 2019·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Yinling JiangShuyu Gui
Oct 16, 2019·Diabetic Medicine : a Journal of the British Diabetic Association·S SalujaA H Heald
Nov 2, 2019·Cytotherapy·Komal Kaushik, Amitava Das
Nov 5, 2019·Trends in Molecular Medicine·Xiaozhen DaiYi Tan
Dec 31, 2019·Journal of Diabetes Research·Wenlong ShangHuiwen Ren
Feb 8, 2020·Cell Biochemistry and Function·Sadegh DehghaniElahe Mahdipour

❮ Previous
Next ❯

Citations

May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ipek SüntarLuciano Saso
Nov 19, 2021·Critical Reviews in Food Science and Nutrition·Yao ChenZhiyong He

❮ Previous
Next ❯

Methods Mentioned

BETA
amputation
density gradient centrifugation
flow cytometry
transfection
biopsy
MDA
FACS
protein assay
electrophoresis
PCR

Software Mentioned

Graphpad Prism
Quantity
FloJo
GraphPad
ImageJ
Image J

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.