DOI: 10.1101/452730Oct 25, 2018Paper

Procyclic Trypanosoma brucei cell cycle is impaired at the G1 stage by the presence of poly(ADP-ribose) in the nucleus

BioRxiv : the Preprint Server for Biology
Mariana SchlesingerSilvia H Fernández Villamil

Abstract

Previously we have demonstrated that an excess of poly (ADP-ribose) in the nucleus makes procyclic parasites more sensitive to hydrogen peroxide. However, the effect of an altered-PAR metabolism under standard conditions has not been addressed yet. Here we have analyzed the behavior of the growth curve of transgenic parasites that present this different PAR metabolism-related phenotypes and studied cell cycle progression in synchronized cultures by flow cytometry and immunofluorescence. We have demonstrated that an excess of nuclear poly (ADP-ribose) produces a delay in the G1 phase of the cell cycle. Moreover, for the first time we have shown that poly (ADP-ribose) occurs at specific points very close to the mature basal body, suggesting there could be a link between the kinetoplast and poly (ADP-ribose) metabolism.

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