PRODAN differentially influences its local environment.

Physical Chemistry Chemical Physics : PCCP
Adam SuhajDylan M Owen

Abstract

Environmentally-sensitive membrane dyes have been extensively used to study the different liquid phases, (liquid-ordered (Lo) and liquid-disordered (Ld)) of the heterogenous cellular membrane. However, it is not yet well understood how these dyes affect membrane properties upon and post insertion. Using a combination of molecular dynamics (MD) simulations and fluorescence microscopy, we study the effect of PRODAN insertion upon its local environment. We firstly present the results of the MD simulations of PRODAN interacting with lipid bilayers of various compositions, specifically the resultant hydration and lipid order of the system. Experimentally, the lipid order of Lo and Ld vesicles containing various concentrations of PRODAN are inferred from their Generalised Polarisation (GP) values, calculated using their fluorescence spectra. We then apply the methodology to a more complex biological system, the HeLa cell line. For both systems, the presence of PRODAN influences its local environment differently between the Lo and Ld phases. In the simulated systems, the presence of PRODAN lowers the lipid order in the Ld phase and increases the order in the Lo phase, whilst experimental data demonstrates that even a small increase in...Continue Reading

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Citations

Aug 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hugo A L FilipeLuís M S Loura
Apr 30, 2019·Physical Chemistry Chemical Physics : PCCP·Paul SmithChristian D Lorenz
Nov 7, 2020·Soft Matter·Sze May YeeChristian D Lorenz
Oct 16, 2021·The Journal of Physical Chemistry Letters·Katarzyna Rybicka-JasińskaValentine I Vullev
Dec 15, 2021·The Journal of Physical Chemistry. B·Paulina GaseckaSophie Brasselet

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Methods Mentioned

BETA
fluorescence microscopy
fluorescence imaging
fluorescence lifetime imaging
NMR
motility studies

Software Mentioned

GraphPad Prism
Origin
GROMACS
PRODAN

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