Producing Coral Offspring with Cryopreserved Sperm: A Tool for Coral Reef Restoration

Scientific Reports
Mary HagedornRebecca Spindler

Abstract

Cryopreservation is an important conservation tool, which may help reef-building coral survive. However, scaling-up from small, laboratory-sized experiments to higher-throughput restoration is a major challenge. To be an effective restoration tool, the cryopreservation methods and husbandry to produce new offspring must be defined. This study examined small and larger-scale in vitro reproduction and settlement for Acropora tenuis and Acropora millepora and found that: 1) cryopreservation of coral sperm reduced sperm motility and fertilization success in half, thus fresh sperm, capable of becoming highly motile, is key; 2) the sperm-to-egg ratio and the concentration of the cryoprotectant treatments affected fertilization success in small- and larger-scale reproduction trials using cryopreserved sperm (p < 0.05); 3) cryopreservation did not affect settlement success, as larvae produced with fresh or cryopreserved sperm had the same settlement success (p > 0.05); and 4) the residence time of the sperm within the bank was not important as the fertilization success of sperm frozen for less than 1 month was similar to that frozen up to 2 years (p > 0.05). These results described the first settlement for coral larvae produced from cr...Continue Reading

References

Dec 21, 2000·ILAR Journal·D Gao, J K Critser
Aug 16, 2003·Science·T P HughesJ Roughgarden
Jul 19, 2008·Science·O Hoegh-GuldbergC D Thomas
Jun 5, 2012·Cryobiology·Mary HagedornRebecca Spindler
Aug 6, 2014·Advances in Experimental Medicine and Biology·Mary Hagedorn, Rebecca Spindler
Jun 5, 2015·Scientific Reports·J TebbenT Harder
Jun 27, 2015·Science·Groves B DixonMikhail V Matz
Sep 29, 2015·Studies in History and Philosophy of Biological and Biomedical Sciences·Sara Peres
Dec 22, 2016·Scientific Reports·Ruben van HooidonkSerge Planes
Mar 2, 2017·Global Change Biology·Madeleine J H van OppenHollie M Putnam

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Citations

Oct 26, 2018·Scientific Reports·Jonathan DalyMary Hagedorn
Feb 23, 2020·Genes·Ben J NovakThomas H Maloney
Aug 2, 2020·Scientific Reports·Carly J RandallAndrew P Negri
Jan 10, 2021·Scientific Reports·Nikolas ZuchowiczMary Hagedorn
Sep 9, 2021·Proceedings of the National Academy of Sciences of the United States of America·Mary HagedornKristen L Marhaver

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Methods Mentioned

BETA
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FSW
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