Product inhibition of the recombinant CelS, an exoglucanase component of the Clostridium thermocellum cellulosome

Applied Microbiology and Biotechnology
K KruusJ H Wu

Abstract

CelS is the most abundant subunit and an exoglucanase component of the Clostridium thermocellum cellulosome, multicomponent cellulase complex. The product inhibition pattern of CelS was examined using purified recombinant CelS (rCelS) produced in Escherichia coli. The rCelS activity on cellopentaose was strongly inhibited by cellobiose. The rCelS activity was also inhibited by lactose. Glucose was only marginally inhibitory. Cellobiose appeared to inhibit the rCelS activity through a competitive mechanism. The inhibition was relieved when beta-glucosidase was added, presumably because of the conversion of cellobiose into glucose. These hydrolysis product inhibition patterns are consistent with those of the crude enzyme (cellulosome), suggesting that CelS is a rate-limiting factor in the activity of the cellulosome.

Citations

Apr 30, 2008·Applied Microbiology and Biotechnology·Kenro TokuhiroHaruo Takahashi
Jan 13, 2009·Journal of Industrial Microbiology & Biotechnology·Arnold L Demain
Jan 12, 2013·Applied Biochemistry and Biotechnology·M López-AbelairasJ M Lema
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Michael NewcombJ H David Wu
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lee R LyndIsak S Pretorius
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Arnold L DemainJ H David Wu
Apr 26, 2001·Analytical Biochemistry·M KitaokaK Hayashi
Jul 29, 2010·Biotechnology Journal·Fahimeh SalimiRadhakrishnan Mahadevan
Oct 15, 2013·FEMS Microbiology Reviews·Sara E Blumer-SchuetteRobert M Kelly
Apr 1, 1999·Biotechnology and Bioengineering·E KimD B Wilson

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