PMID: 375985May 10, 1979Paper

Product inhibition studies of yeast phosphoglycerate kinase evaluating properties of multiple substrate binding sites

Biochimica Et Biophysica Acta
B Schierbeck, M Larsson-Raźnikiewicz

Abstract

Product inhibition studies on yeast phosphoglycerate kinase (ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) have been performed with 1,3-P2-glycerate. The results indicate that: 1. The catalytic reaction can be affected via four substrate binding sites, two for MgATP2- and two for 3-P-glycerate. 2. There is one catalytic centre per enzyme molecule. 3. The catalytic reaction primarily occurs at the 'first' or 'high affinity' MgATP2- and 3-P-glycerate binding sites. The 'second' set of sub-sites for these substrates are located in a region for regulation of the catalytic reaction. 4. The products of the reaction, 1,3-P2-glycerate and ADP, are preferentially bound to the regulatory region. 5. MgATP2- and 1,3-P2-glycerate are able to bind simultaneously to this region. When liganded with MgATP2- the apparent Ki value for 1,3-P2-glycerate increases from 3 microM to 20 microM.

References

Mar 16, 1976·European Journal of Biochemistry·P TanswellR J Williams
Apr 17, 1978·European Journal of Biochemistry·J A Wrobel, R A Stinson
Feb 1, 1976·The Biochemical Journal·S P SpraggW A Barnett
Mar 15, 1977·Biochimica Et Biophysica Acta·M L Raźnikiewicz, B Schierbeck
Jan 1, 1976·Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry·L ArvidssonM Larsson-Raźnikiewicz
Aug 12, 1976·Biochimica Et Biophysica Acta·M Ali, Y S Brownstone
Apr 8, 1974·Biochemical and Biophysical Research Communications·M Larsson-Raźnikiewicz, B Schierbeck
Jan 4, 1974·Nature·T N BryantP L Wendell
May 1, 1974·FEBS Letters·B E ChapmanG H Reed
Nov 1, 1974·FEBS Letters·P TanswellR J Williams
Oct 26, 1971·European Journal of Biochemistry·M Larsson-Raźnikiewicz, L Arvidsson
Nov 1, 1970·European Journal of Biochemistry·M Larsson-Raźnikiewicz
Feb 1, 1973·FEBS Letters·M Larsson-Raźnikiewicz, J R. Jansson

❮ Previous
Next ❯

Citations

Jun 1, 1996·Protein Science : a Publication of the Protein Society·C W Cheung, M T Mas
Jan 1, 1986·Journal of Inherited Metabolic Disease·L G SvirklysW J O'Sullivan
May 29, 2009·Journal of the Royal Society, Interface·Christoph Hold, Sven Panke
Jun 20, 1990·European Journal of Biochemistry·W J FairbrotherR J Williams
Apr 10, 1991·European Journal of Biochemistry·H C Graham, R J Williams
Aug 15, 1993·European Journal of Biochemistry·H C João, R J Williams
Jan 15, 1981·Biochimica Et Biophysica Acta·M M Khamis, M Larsson-Raźnikiewicz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.