Production and application of anti-nucleoprotein IgY antibodies for influenza A virus detection in swine

Journal of Immunological Methods
Miriele Caroline da SilvaEmerson José Venancio

Abstract

Influenza A virus (IAV) causes an important respiratory disease in mammals and birds leading to concerns in animal production industry and public health. Usually, antibodies produced in mammals are employed in diagnostic tests. However, due to animal welfare concerns, technical advantages and the high cost of production, alternatives to the production of antibodies in mammals have been investigated. The aim of this study was to produce egg yolk immunoglobulin (IgY) in laying hens against a highly conserved protein (nucleoprotein- NP) of IAV and to evaluate the application of anti-NP IgY antibodies in virus detection by immunocytochemistry (ICC) and immunohistochemistry (IHC). Three laying hens of the White Leghorn line were inoculated seven times with a recombinant NP protein and their eggs collected seven days after the 3rd, 5th and 7th inoculations. Immunoglobulin Y antibodies were purified from egg yolk through precipitation with ammonium sulfate. The titers and specificity of the purified antibodies were determined by ELISA, western blotting, ICC and IHC. High levels of specific anti-NP antibodies were detected by ELISA after the 5th inoculation, reaching a peak after the 7th inoculation. The mean yield of total protein in ...Continue Reading

Citations

Jun 17, 2020·Experimental and Therapeutic Medicine·Carolina ConstantinDemetrios A Spandidos
May 1, 2021·International Journal of Molecular Sciences·Pharaoh Fellow MwaleYi-Yuan Yang
Jun 29, 2021·Frontiers in Immunology·Lucia LeeDaria Mochly-Rosen

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