PMID: 7524240Jun 1, 1994Paper

Production and application of monoclonal antibodies to ovine interleukin-1 alpha and interleukin-1 beta

Veterinary Immunology and Immunopathology
P J EganA D Nash

Abstract

Monoclonal antibodies (mAbs) were raised against recombinant ovine interleukin-1 alpha and beta (ovIL-1 alpha and ovIL-1 beta). Five ovIL-1 alpha specific mAbs and three ovIL-1 beta specific mAbs, all of the IgG1 isotype, were characterized. Four of the five ovIL-1 alpha specific mAbs, designated 10.36, 10.49, 10.82 and 5.16, fell into two distinct groups based on several criteria. MAbs 10.36, 10.49 and 10.82 reacted with recombinant ovIL-1 alpha in Western blot analysis, were potent in neutralizing ovIL-1 alpha biological activity in vitro and bound to the same or a closely related epitope. MAb 5.16 also bound ovIL-1 alpha in Western blot analysis, but was less potent in neutralizing ovIL-1 alpha biological activity and bound to a different epitope. A fifth ovIL-1 alpha specific mAb, 5.01, had some characteristics of antibodies from both groups. While the combination of mAb 5.16 with any of 10.36, 10.49 and 10.82 was suitable for detection of ovIL-1 alpha in a sandwich immunoassay, the most sensitive detection of ovIL-1 alpha utilized mAb 10.82 for capture and a rabbit polyclonal anti-ovIL-1 alpha antiserum as the detecting antibody in combination with a HRPO-conjugated anti-rabbit Ig reagent. This combination of reagents had ...Continue Reading

References

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Citations

Jan 1, 1996·Veterinary Immunology and Immunopathology·M L CrossG S Buchan
Jun 20, 2003·Veterinary Immunology and Immunopathology·Reji PaalangaraPeter McCullagh
Nov 1, 1996·Veterinary Immunology and Immunopathology·A D NashV M Bowles
Jul 1, 1997·Veterinary Immunology and Immunopathology·J S RothelP R Wood

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