PMID: 8467999Feb 15, 1993Paper

Production and characterisation of a Legionella pneumophila specific monoclonal antibody

FEMS Microbiology Letters
M S Lever

Abstract

To better define the antigenic structure of the outer cell membranes of Legionellae, a panel of 6 monoclonal antibodies was raised against partially purified outer membranes of Legionella pneumophila serogroup 1, Corby strain. This study describes the purification and characterization of one of these monoclonal antibodies reacting with a 135-kDa protein, which was shown to be common to all 14 serogroups of Legionella pneumophila. It shows no cross-reactivity with 20 other Legionella species, or 9 other Gram-negative species tested by SDS-PAGE and Western blotting procedures. The epitope would appear to be predominantly surface exposed and, from preliminary detergent extraction studies, not peptidoglycan-associated.

Citations

Sep 9, 2008·Sexual Abuse : a Journal of Research and Treatment·Julia E McLawsenMario J Scalora

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