Abstract
Fourteen hybridoma cell lines were isolated that produced monoclonal antibodies (MAbs) to purified Verotoxins 1 and 2 (VT1, VT2) of Escherichia coli of serotype O157:H7. Of these MAbs, eight were obtained by immunisation of BALB/c mice with purified VT1, and six were obtained from BALB/c mice immunised with purified VT2. With the exception of MAb 1C5, with a heavy chain of IgG2b class, antibodies produced from mice immunised with heat-treated toxin were of IgM class. MAbs produced from mice immunised with heat-treated VT1 or VT2 reacted with both verotoxins in ELISA, and Western-blot analysis revealed that they reacted with subunit A and the A1 fragment of nicked subunit A of both toxins, but not with subunit B; furthermore, none of them neutralised Vero cytotoxicity or mouse lethality of either toxin. In contrast, MAbs produced from mice immunised with heat-treated and formalin-treated VT1 reacted in Western blots with subunits A and B of VT1 and subunit A, but not subunit B, of VT2, reacted in ELISA with VT1 only, and neutralised Vero cytotoxicity and mouse lethality of VT1 but not of VT2. Results indicate the existence of a common epitope on subunit A of VT1 and VT2 that is not responsible for the biological activity of thes...Continue Reading
Citations
May 9, 2001·Blood·D KarpmanS Lethagen
Jul 24, 2012·Toxins·Siu-Kei Chow, Arturo Casadevall
May 26, 2016·Scientific Reports·Geoffrey MaindaDavid L Gally
Jul 26, 2005·Infection and Immunity·Abhineet S SheoranSaul Tzipori
May 23, 2003·Infection and Immunity·Abhineet S SheoranSaul Tzipori
Oct 25, 2013·Toxins·Luisa W ChengXiaohua He
Jan 18, 2002·Infection and Immunity·Jean MukherjeeSaul Tzipori
Oct 19, 2004·Clinical Microbiology Reviews·Saul TziporiHoward Trachtman
Sep 14, 2002·Infection and Immunity·Jean MukherjeeSaul Tzipori
Apr 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sharon X WenAlison D O'Brien
Jun 28, 2011·BMC Microbiology·Ziad W JaradatArun K Bhunia
Jun 24, 2005·Infection and Immunity·D E AkiyoshiS Tzipori
Feb 19, 2002·Journal of Food Protection·T ZhaoScott Ladely
Oct 26, 1999·Infection and Immunity·H NakaoT Takeda
Apr 1, 1995·Applied and Environmental Microbiology·T ZhaoL Garber
Jan 1, 1991·Journal of Clinical Microbiology·N V Padhye, M P Doyle
Jul 1, 1996·Applied and Environmental Microbiology·G WangM P Doyle
Oct 1, 1991·Applied and Environmental Microbiology·R D Weeratna, M P Doyle
Sep 1, 1996·Applied and Environmental Microbiology·Y HeJ Kwang