Production and characterisation of recombinant forms of human pulmonary surfactant protein C (SP-C): Structure and surface activity

Biochimica Et Biophysica Acta
Dunja LukovicIsmael Mingarro

Abstract

Surfactant protein C (SP-C) is an essential component for the surface tension-lowering activity of the pulmonary surfactant system. It contains a valine-rich alpha helix that spans the lipid bilayer, and is one of the most hydrophobic proteins known so far. SP-C is also an essential component of various surfactant preparations of animal origin currently used to treat neonatal respiratory distress syndrome (NRDS) in preterm infants. The limited supply of this material and the risk of transmission of infectious agents and immunological reactions have prompted the development of synthetic SP-C-derived peptides or recombinant humanized SP-C for inclusion in new preparations for therapeutic use. We describe herein the recombinant production in bacterial cultures of SP-C variants containing phenylalanines instead of the palmitoylated cysteines of the native protein, as fusions to the hydrophilic nuclease A (SN) from Staphylococcus aureus. The resulting chimerae were partially purified by affinity chromatography and subsequently subjected to protease digestion. The SP-C forms were recovered from the digestion mixtures by organic extraction and further purified by size exclusion chromatography. The two recombinant SP-C variants so obta...Continue Reading

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Citations

Oct 7, 2008·Accounts of Chemical Research·Nathan J BrownAnnelise E Barron
Oct 14, 2014·Biochimica Et Biophysica Acta·Nuria RoldanBegoña Garcia-Alvarez
May 6, 2010·Biochimica Et Biophysica Acta·Michelle T DohmAnnelise E Barron
Aug 9, 2011·Biochimica Et Biophysica Acta·Clara Aicart-RamosIgnacio Rodriguez-Crespo
Oct 21, 2016·Biophysical Journal·Nuria RoldanBegoña García-Álvarez
May 24, 2017·Nature Communications·Nina KronqvistJan Johansson
Apr 26, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Dunja LukovicJesus Pérez-Gil
Dec 26, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Aranzazu MatoM Auxiliadora Prieto

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