Production and characterization of antibodies against crosslinked gelatin nanoparticles and first steps toward developing an ELISA screening kit

Analytical and Bioanalytical Chemistry
Vincent DehaluPhilippe Delahaut

Abstract

Nanotechnologies are finding a growing range of applications in the food sector. Nanoparticles are used notably to add vitamins and other nutrients to foods and beverages without affecting taste and color. They are also used to develop new tastes, preserve food texture, control the release of flavors, improve the bioavailability of compounds such as antioxidants and vitamins, and monitor freshness with nanosensors. Crosslinked gelatin nanoparticles are a component of nano-sized carriers for nutrient and supplement delivery in foods and related products. This paper describes the production and characterization of polyclonal antibodies against gelatin nanoparticles. Two immunization schemes were investigated: subcutaneous injection with and without a first intravenous injection. Two enzyme-linked immunosorbent assay formats were used to characterize the antibodies: an inhibition format with an antigen-coated plate for detection of the immune response and a sandwich format for development of the method. The antibodies showed good sensitivity with an IC50 equal to 0.11 ng mL(-1) using indirect ELISA format and a good specificity for the nanomaterials, without significant cross-reactivity against native gelatin. The limit of detecti...Continue Reading

References

Mar 4, 2008·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Qasim ChaudhryRichard Watkins
Sep 2, 2008·Journal of Agricultural and Food Chemistry·Dion M A M LuykxHans Bouwmeester

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Citations

Apr 8, 2016·Critical Reviews in Food Science and Nutrition·Aditi JainChidambaram Ramalingam
Mar 22, 2018·Journal of Biomaterials Science. Polymer Edition·Janicy Arantes CarvalhoAndreza Ribeiro Simioni
Jul 1, 2014·Comprehensive Reviews in Food Science and Food Safety·Gurmit SinghTimothy V Duncan
Jul 1, 2014·Comprehensive Reviews in Food Science and Food Safety·Christopher SzakalTimothy V Duncan

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