Production and characterization of auxin-insensitive rice by overexpression of a mutagenized rice IAA protein

The Plant Journal : for Cell and Molecular Biology
Ayako NakamuraMakoto Matsuoka

Abstract

Since auxin was first isolated and characterized as a plant hormone, the underlying molecular mechanism of auxin signaling has been elucidated primarily in dicot plants represented by Arabidopsis. In monocot plants, the molecular mechanism of auxin signaling has remained unclear, despite various physiological experiments. To understand the function and mechanism of auxin signaling in rice (Oryza sativa), we focused on the IAA gene, a well-studied gene in Arabidopsis that serves as a negative regulator of auxin signaling. We found 24 IAA gene family members in the rice genome. OsIAA3 is one of these family members whose expression is rapidly increased in response to auxin. We produced transgenic rice harboring mOsIAA3-GR, which can overproduce mutant OsIAA3 protein containing an amino acid change in domain II to cause a gain-of-function phenotype, by treatment with dexamethasone. The transgenic rice was insensitive to auxin and gravitropic stimuli, and exhibited short leaf blades, reduced crown root formation, and abnormal leaf formation. These results suggest that, in rice, auxin is important for development and its signaling is mediated by IAA genes.

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Citations

Feb 9, 2012·Molecular Genetics and Genomics : MGG·Jian WuGang Lu
Mar 20, 2010·Cold Spring Harbor Perspectives in Biology·Paula McSteen
Jan 16, 2007·Plant Physiology·Yuko YamamotoTakashi Sazuka
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Mar 22, 2015·Journal of Experimental Botany·Wanneng YangLizhong Xiong
Jan 26, 2013·The Plant Journal : for Cell and Molecular Biology·Xinai ChenChuanzao Mao
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Aug 8, 2019·Plant Biotechnology Journal·Chanjuan MaoFeng Ming
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Dec 30, 2020·Plants·Mallana Gowdra MallikarjunaHari Shanker Gupta
Dec 7, 2021·Frontiers in Plant Science·Feibing WangXinhong Chen

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