PMID: 9444984Jan 28, 1998Paper

Production and characterization of improved adenovirus vectors with the E1, E2b, and E3 genes deleted

Journal of Virology
Andrea AmalfitanoJ S Chamberlain

Abstract

Adenovirus (Ad)-based vectors have great potential for use in the gene therapy of multiple diseases, both genetic and nongenetic. While capable of transducing both dividing and quiescent cells efficiently, Ad vectors have been limited by a number of problems. Most Ad vectors are engineered such that a transgene replaces the Ad E1a, E1b, and E3 genes; subsequently the replication-defective vector can be propagated only in human 293 cells that supply the deleted E1 gene functions in trans. Unfortunately, the use of high titers of E1-deleted vectors has been repeatedly demonstrated to result in low-level expression of viral genes still resident in the vector. In addition, the generation of replication-competent Ad (RCA) by recombination events with the E1 sequences residing in 293 cells further limits the usefulness of E1-deleted Ad vectors. We addressed these problems by isolating new Ad vectors deleted for the E1, E3, and the E2b gene functions. The new vectors can be readily grown to high titers and have several improvements, including an increased carrying capacity and a theoretically decreased risk for generating RCA. We have also demonstrated that the further block to Ad vector replication afforded by the deletion of both th...Continue Reading

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Citations

Oct 12, 1999·Gene Therapy·A Amalfitano
Nov 10, 2001·Annual Review of Genomics and Human Genetics·A Pfeifer, I M Verma
Sep 11, 2002·The Journal of Gene Medicine·Heshan Sam ZhouArthur L Beaudet
Jan 15, 2004·American Journal of Respiratory Medicine : Drugs, Devices, and Other Interventions·Ajay GautamCharles L Densmore
Sep 19, 2014·Clinical and Vaccine Immunology : CVI·Benjamin J KoestlerChristopher M Waters
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·O CortiJ Mallet
Jun 10, 2006·The Journal of Pharmacology and Experimental Therapeutics·Kristini K MilesJoseph K Ritter
Jul 23, 2008·The Journal of Pharmacy and Pharmacology·Helen O McCarthyDavid G Hirst
Dec 22, 2004·Human Gene Therapy·Michael J McConnell, Michael J Imperiale
Oct 19, 2005·Gene Therapy·R AlbaM Chillon
Jul 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniel M AppledornAndrea Amalfitano
Sep 3, 2010·The Journal of Clinical Investigation·Anneke I den HollanderFrans P M Cremers
Mar 20, 2020·Physiological Reviews·Sonja KleinlogelTobias Moser
May 28, 2020·Cold Spring Harbor Protocols·Miguel Sena-Esteves, Guangping Gao
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·A AmalfitanoY T Chen
Oct 20, 2000·The Journal of General Virology·W C Russell
Oct 3, 2001·Journal of Virology·W ZhangM J Imperiale
Jan 9, 2003·Expert Opinion on Biological Therapy·P J WysockiA Mackiewicz
Jul 26, 2003·Expert Opinion on Biological Therapy·Paul Gregorevic, Jeffrey S Chamberlain
May 20, 2006·Reviews in Medical Virology·Manuel A F V Gonçalves, Antoine A F de Vries
Apr 3, 2010·Cancer Immunology, Immunotherapy : CII·Elizabeth S GabitzschFrank R Jones
Dec 16, 2006·International Reviews of Immunology·Takuya OsadaH Kim Lyerly
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Dec 20, 2005·The Journal of Pathology·Lawrence S YoungVivien Mautner
Jan 4, 2013·PloS One·Patrick J AtkinsonRachael T Richardson

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