PMID: 7091814Jan 1, 1982Paper

Production and characterization of monoclonal antibodies to bovine immunoglobulin G2

American Journal of Veterinary Research
S SrikumaranR A Goldsby

Abstract

Spleen cells from Balb/cJ mice inoculated with bovine immunoglobulin (Ig)G were hybridized with hypoxanthine aminopterin thymidine-sensitive nonsecreting cell line SP-2/0, and the hybrid cell culture fluid was tested for specificity. Hybrid cells secreting monoclonal antibodies to bovine IgG were isolated and recloned. The monoclonal antibody DAS 2 was specific for bovine IgG2 by an indirect solid-phase radioimmunoassay. This clone was adapted for growth as antibody-secreting neoplasms in Balb/cJ mice, the sera from which contained high titers (1:128,000) of anti-bovine IgG2 antibodies. Antibody specific to bovine IgG2 was isolated by affinity chromatography. This antibody was shown by Ouchterlony analysis to be mouse IgG1 with the kappa light chain. On isoelectric focusing, this antibody gave a pattern that was consistent with monoclonality.

Related Concepts

Monoclonal Antibodies
Bos indicus
Cell Fusion Procedure
Hybridomas
Polyglobin
Mice, Inbred Strains
Neoplasm Transplantation
Neoplasms, Experimental
Radioimmunoassay
Spleen

Related Feeds

Antibody Engineering

Antibody engineering technologies are constantly advancing to improve the clinical effectiveness of monoclonal and bispecific antibodies. Discover the latest research on Antibody Engineering here.