PMID: 7519581Apr 1, 1994Paper

Production and characterization of monoclonal antibodies against the ribosome inactivating proteins dianthin32 and momochin

Hybridoma
G PorroD Modena

Abstract

Female BALB/c mice were immunized with either dianthin32 or momochin, type 1 ribosome-inactivating proteins (RIPs) derived from Dianthus charyophyllus and Momordica cochinchinensis, respectively. Five anti-dianthin32 and 6 anti-momochin secreting hybridomas were obtained by somatic fusion of lymphocytes with myeloma cell line NS0. The monoclonal antibodies (MAbs) produced were highly specific, as demonstrated by cross-reactivity assays performed with taxonomically related and unrelated type 1 RIPs, and recognized different epitopes of the antigen. The affinity constant of anti-RIPs MAbs ranged between 10(8) M-1 and 10(10) M-1.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 19, 1992·Journal of Immunological Methods·P StrocchiF Stirpe
Oct 1, 1991·British Journal of Cancer·E J Wawrzynczak
Jan 24, 1991·Nature·G Winter, C Milstein
Feb 1, 1991·Hybridoma·J Zimmermann, W E Trommer
Jan 1, 1990·Progress in Growth Factor Research·D A Lappi, A Baird
Dec 1, 1989·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A A Hertler, A E Frankel
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M S McGrathJ Marsh
May 4, 1987·Journal of Immunological Methods·R ComittiY M Galante
Sep 1, 1983·European Journal of Immunology·J A SchroerK J Kim
Jan 1, 1983·International Journal of Immunopharmacology·F SpreaficoF Stirpe

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