Production and characterization of monoclonal antibodies against mink leukocytes

Veterinary Immunology and Immunopathology
W ChenB Aasted

Abstract

Three monoclonal antibodies (mAbs) were generated against mink leukocytes. One antibody reacted with all T lymphocytes, one with all monocytes and one had platelet reactivity. Under reducing conditions, the T lymphocyte reactive antibody immunoprecipitated 18 kDa, 23 kDa, 25 kDa and 32-40 kDa polypeptides and the platelet reactive antibody 17 kDa, 22 kDa plus two high molecular weight (> 100 kDa) polypeptides. Immunohistological studies of the mAbs were performed in order to localize the cellular distribution of the detected antigens in various organs. The T lymphocyte reactive antibody detected an antigen, which was widely distributed in the T cell area of lymph nodes and spleen and in the thymic medulla. We conclude that this antibody is an anti-CD3 mAb and suggest that the platelet reactive antibody reacts to the CD41/CD61 integrin molecule. In addition to our own mAbs, more than 100 mAbs against leukocytes of human and various animal species have been analysed for cross-reactivity to mink leukocytes. We found eight to cross-react with mink. Of particular importance was an anticanine CD11a mAb, an antihuman CD79a mAb and an antihuman bcl-2 mAb.

References

Mar 1, 1989·Veterinary Immunology and Immunopathology·B Aasted
Jul 1, 1988·Veterinary Immunology and Immunopathology·B AastedA Uttenthal
Dec 1, 1993·Veterinary Immunology and Immunopathology·C N JacobsenJ L Petersen
May 20, 1998·Veterinary Immunology and Immunopathology·B AastedF Zuckermann

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Citations

Oct 5, 2002·Journal of Anatomy·Julie M Old, Elizabeth M Deane
Jun 14, 2003·Journal of Virology·P V JensenB Aasted
May 20, 2008·Journal of Immunological Methods·John A RutiglianoPaul G Thomas
Aug 8, 2007·Veterinary Immunology and Immunopathology·B Aasted, B Viuff
Sep 8, 2004·Veterinary Immunology and Immunopathology·Julie A SchwartzF Charles Mohr
Jan 25, 2000·The Journal of General Virology·K T JensenM E Bloom
Jun 6, 2009·The Journal of General Virology·Line NielsenMerete Blixenkrone-Møller
Oct 3, 1999·Allergy·M L Díez-GómezA Martínez

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