PMID: 7537335Sep 1, 1994Paper

Production and characterization of murine monoclonal antibodies specific for baboon IgG heavy and light chain epitopes

Journal of Medical Primatology
M H ShearerR C Kennedy

Abstract

We have generated a panel of murine monoclonal antibodies (MAbs) that recognize baboon IgG epitopes. The reactivity of the MAbs with IgG from other primate species was also examined. Specificity for IgG heavy (H) or light (L) chain epitopes was determined by Western blot analysis. The H chain-specific MAbs were analyzed for IgG subclass specificity and the L chain-specific MAbs for reactivity with baboon IgM and polymeric sIgA. Finally, an ELISA was developed to demonstrate the utility of the MAbs in analysis of humoral immune responses in baboons.

References

Jun 1, 1991·Molecular Immunology·W A HookR P Siraganian
Aug 1, 1971·Teratology·J A Bollert, A G Hendrickx
Jan 1, 1980·Methods of Biochemical Analysis·E A Bayer, M Wilchek

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Citations

May 12, 1997·Journal of Immunological Methods·M H ShearerR C Kennedy
Dec 15, 2004·Journal of Reproductive Immunology·Luis D GiavedoniPeter W Nathanielsz
Aug 15, 2006·Virology·Roman F WolfDirk P Dittmer

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