Production and characterization of Newcastle disease antibody as a reagent to develop a rapid immunodiagnostic test tool

Veterinary World
Dwi Desmiyeni PutriOkti Nadia Poetri

Abstract

This research was conducted to produce and characterize ND antibody as reagent candidate to develop a rapid immunodiagnostic test tool. Four New Zealand White rabbits were used in this study and divided into two groups. First group was injected by Sato ND antigen, and second group was injected by genotype VII ND antigen. This study is divided into three steps: (a) ND antibody production, (b) ND antibody purification, and (c) ND antibody characterization. First group was rabbit injected by Sato NDV (5×108.25 egg lethal doses (ELD)50/ml) and second group was injected by genotype VII NDV (5×106.5 ELD50/ml). Antigen induction was performed by subcutaneous administrated for first (day 1) and second (day 14) injection and intravenous administrated for third (day 30) injection. Blood was collected on day 8 after third injection. Antibody production increased on second antigen injection and reached a peak on day 9 after second antigen injection. Sato and genotype VII ND antibody can be produced without adjuvant within 38 days with the highest titer 210. Based on antibody titer data, both antigens induced antibody production in a similar trend. The characterization antibody by SDS-PAGE indicated that molecular weight of immunoglobulin G...Continue Reading

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Citations

Jun 14, 2019·Veterinary World·Melani Wahyu AdiningsihOkti Nadia Poetri
Sep 4, 2021·Veterinary World·Charlie Franck Arthur N'Guessan AmoiaEmmanuel Couacy-Hymann

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Methods Mentioned

BETA
Assay
electrophoresis
antibody purification

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