Production and characterization of oil-in-water emulsions containing droplets stabilized by beta-lactoglobulin-pectin membranes

Journal of Agricultural and Food Chemistry
Lydie MoreauDavid J McClements

Abstract

Oil-in-water emulsions containing droplets stabilized by beta-lactoglobulin (beta-Lg)-pectin membranes were produced using a two-stage process. A primary emulsion containing small droplets (d(32) approximately 0.3 microm) was prepared by homogenizing 10 wt % corn oil with 90 wt % aqueous solution (1 wt % beta-Lg, 5 mM imidazole/acetate buffer, pH 3.0) using a high-pressure valve homogenizer. The primary emulsion was then diluted with pectin solutions to produce secondary emulsions with a range of pectin concentrations (5 wt % corn oil, 0.45 wt % beta-Lg, 5 mM imidazole/acetate buffer, 0-0.22 wt % pectin, pH 3.0). The electrical charge on the droplets in the secondary emulsions decreased from +33 +/- 3 to -19 +/- 1 mV as the pectin concentration was increased from 0 to 0.22 wt %, which indicated that pectin adsorbed to the droplet surfaces. The mean particle diameter of the secondary emulsions was small (d(32) < 1 microm) at relatively low pectin concentrations (<0.04 wt %), but increased dramatically at higher pectin concentrations (e.g., d(32) approximately 13 microm at 0.1 wt % pectin), which was attributed to charge neutralization and bridging flocculation effects. Emulsions with relatively small mean particle diameters (d(3...Continue Reading

Citations

Jun 2, 2009·Critical Reviews in Food Science and Nutrition·David Julian McClementsJochen Weiss
Mar 30, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Yan Li, David Julian McClements
May 18, 2010·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Yan LiDavid Julian McClements
Feb 22, 2013·Journal of the Science of Food and Agriculture·Yankun Zhou, Yrjö H Roos
May 15, 2012·Journal of Colloid and Interface Science·Aiqian YeHarjinder Singh
Jun 23, 2016·Journal of Agricultural and Food Chemistry·Andrea Alvarez-SalaMaría Jesús Lagarda
Dec 18, 2016·Colloids and Surfaces. B, Biointerfaces·María I Moran-ValeroAna M R Pilosof
Feb 24, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bulei ShengHui Rong
Nov 13, 2007·Journal of Food Science·D J McClementsJ Weiss
May 25, 2010·Journal of Food Science·David Julian McClements
Jan 11, 2017·AAPS PharmSciTech·Atinderpal KaurShweta Dang
Jun 3, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Keke Xu, Ping Yao
Jan 10, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Horst AdelmannRaffaele Mezzenga
Oct 12, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Raffaele Mezzenga, Stephane Ulrich
Aug 23, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Yeun Suk GuD Julian McClements
Feb 29, 2008·Journal of Agricultural and Food Chemistry·Daigo IwanagaDavid Julian McClements
Oct 20, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Yeun Suk GuD Julian McClements
Feb 27, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Young-Hee Cho, David Julian McClements
Jun 28, 2008·Journal of Agricultural and Food Chemistry·Marly S KatsudaEric A Decker
Sep 1, 2005·Journal of Agricultural and Food Chemistry·Stefano Tiziani, Yael Vodovotz

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