Production and characterization of poly(3-hydroxypropionate-co-4-hydroxybutyrate) with fully controllable structures by recombinant Escherichia coli containing an engineered pathway

Metabolic Engineering
Dechuan MengGuo-Qiang Chen

Abstract

Copolyesters of 3-hydroxypropionate (3HP) and 4-hydroxybutyrate (4HB), abbreviated as P(3HP-co-4HB), was synthesized by Escherichia coli harboring a synthetic pathway consisting of five heterologous genes including orfZ encoding 4-hydroxybutyrate-coenzyme A transferase from Clostridium kluyveri, pcs' encoding the ACS domain of tri-functional propionyl-CoA ligase (PCS) from Chloroflexus aurantiacus, dhaT and aldD encoding dehydratase and aldehyde dehydrogenase from Pseudomonas putida KT2442, and phaC1 encoding PHA synthase from Ralstonia eutropha. When grown on mixtures of 1,3-propanediol (PDO) and 1,4-butanediol (BDO), compositions of 4HB in microbial P(3HP-co-4HB) were controllable ranging from 12 mol% to 82 mol% depending on PDO/BDO ratios. Nuclear magnetic resonance (NMR) spectra clearly indicated the polymers were random copolymers of 3HP and 4HB. Their mechanical and thermal properties showed obvious changes depending on the monomer ratios. Morphologically, P(3HP-co-4HB) films only became fully transparent when monomer 4HB content was around 67 mol%. For the first time, P(3HP-co-4HB) with adjustable monomer ratios were produced and characterized.

References

May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Aug 15, 1997·FEMS Microbiology Letters·S HeinA Steinbüchel
Nov 10, 1998·Trends in Biotechnology·A Steinbüchel, B Füchtenbusch
Jun 11, 1999·Current Opinion in Biotechnology·B Witholt, B Kessler
Sep 5, 2003·The Biochemical Journal·Bernd H A Rehm
Oct 29, 2003·Current Opinion in Biotechnology·Charles E Nakamura, Gregory M Whited
Apr 1, 2004·Applied Biochemistry and Biotechnology·Tracy M CaroleMark D Paster
Feb 9, 2005·Applied Microbiology and Biotechnology·Guo-Qiang Chen, Qiong Wu
Jun 21, 2007·Journal of Applied Microbiology·R A J VerlindenI Radecka
Jul 23, 2009·Chemical Society Reviews·Guo-Qiang Chen
Aug 12, 2009·Biotechnology Advances·R K SaxenaJasmine Isar
Nov 3, 2009·Metabolic Engineering·Keith E J TyoGregory Stephanopoulos
Apr 7, 2010·Biotechnology Advances·E Celińska
Jun 15, 2010·Applied and Environmental Microbiology·Björn Andreessen, Alexander Steinbüchel
Nov 23, 2010·Chemical Society Reviews·Cheng-Kang ChiangHuan-Tsung Chang
Dec 25, 2010·Applied Microbiology and Biotechnology·Shi Yan LiGuo-Qiang Chen
Feb 1, 2011·Metabolic Engineering·Nanette R Boyle, John A Morgan
Feb 18, 2011·Biotechnology Journal·Anastasia KrivoruchkoJens Nielsen
Mar 23, 2011·Current Opinion in Biotechnology·Jeong Wook LeeSang Yup Lee
May 13, 2011·Trends in Biotechnology·Jeong Wook LeeSang Yup Lee
May 24, 2011·Nature Chemical Biology·Harry YimStephen Van Dien
Jun 18, 2011·Bioresource Technology·Dan TanGuo-Qiang Chen
Jun 28, 2011·Current Opinion in Biotechnology·Xue GaoGuo-Qiang Chen
Dec 14, 2011·Nature Biotechnology·Deepak Dugar, Gregory Stephanopoulos

❮ Previous
Next ❯

Citations

Feb 26, 2014·Metabolic Engineering·Xiao-Zhi FuGuo-Qiang Chen
May 27, 2014·Applied Microbiology and Biotechnology·Björn AndreeßenAlexander Steinbüchel
Mar 12, 2013·Applied Microbiology and Biotechnology·Qian WangQingsheng Qi
Nov 13, 2012·Metabolic Engineering·Daniel E AgnewBrian F Pfleger
Mar 19, 2014·Current Opinion in Biotechnology·De-Chuan MengGuo-Qiang Chen
Sep 28, 2015·Trends in Biotechnology·Guo-Qiang ChenJianwen Ye
Jul 1, 2014·Current Opinion in Biotechnology·Ying WangGuo-Qiang Chen
Jun 18, 2017·Applied Microbiology and Biotechnology·Dan LiGuo-Qiang Chen
Aug 26, 2014·Applied and Environmental Microbiology·Björn AndreessenAlexander Steinbüchel
Aug 11, 2020·Physical Chemistry Chemical Physics : PCCP·Karteek K BejagamGhanshyam Pilania
Apr 7, 2020·Advanced Materials·So Young ChoiSang Yup Lee
Nov 20, 2016·Microbial Biotechnology·Yilin RenGuo-Qiang Chen
Jan 17, 2021·Polymers·Shahina RiazSoo Jin Park
Jan 22, 2021·FEMS Microbiology Reviews·April M LewisRobert M Kelly
Feb 16, 2021·Frontiers in Bioengineering and Biotechnology·Rosa TurcoMartino Di Serio
Mar 10, 2021·Nature Communications·Xiao-Ran JiangGuo-Qiang Chen
Jun 16, 2021·World Journal of Microbiology & Biotechnology·Peng Zhao, Pingfang Tian
Jun 22, 2021·Frontiers in Bioengineering and Biotechnology·Thanawat BoontipSuchada Chanprateep Napathorn
Jul 30, 2021·International Journal of Biological Macromolecules·Rajendran MuthurajTizazu H Mekonnen
Apr 28, 2020·Macromolecules·Grant W FahnhorstThomas R Hoye
Nov 8, 2019·Journal of Chemical Information and Modeling·Ghanshyam PilaniaBabetta L Marrone

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.