Production and characterization of poly(I):poly(C)-induced rat interferons in vitro

Journal of Interferon Research
D L Wiedbrauk, G R Burleson

Abstract

The synthetic polyribonucleotide polyinosinate:polycytidylate [poly(I):poly(C)] was used to induce interferon (IFN) production in a rat leiomyosarcoma cell line. Three separate IFN species were distinguished by antiviral activity on homologous and heterologous cells, interaction with Blue Sepharose CL-6B chromatography matrix, and antibody neutralization studies. Two IFN species, one with a weakly ionic and the other with a hydrophobic interaction with Blue Sepharose, exhibited heterologous antiviral activity, and were acid- and heat-labile. Both had a molecular weight of 30,000 daltons by HPLC-based molecular exclusion chromatography. The weakly ionic species was neutralized only by antiserum to L-cell IFN, whereas the hydrophobic species was neutralized by antiserum to L-cell IFN and antiserum to human leukocyte IFN. The remaining IFN species, with a weakly ionic interaction with Blue Sepharose, was acid- and heat-stable, exhibited no heterologous cell antiviral activity, and was not neutralized by any antisera to IFN. This species had a molecular weight of 39,000 daltons by HPLC-based molecular exclusion chromatography and 34,000 daltons by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These rat fibroblast IFNs ...Continue Reading

References

May 1, 1977·Analytical Biochemistry·J J Sedmak, S E Grossberg
Aug 1, 1978·Virology·E A HavellJ Vilcek
Sep 1, 1974·Virology·W E Stewart
Sep 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·A K FieldM R Hilleman
Apr 1, 1971·Proceedings of the Society for Experimental Biology and Medicine·F DianzaniH B Levy
Jul 10, 1980·Nature
Feb 1, 1981·Journal of Virology·S RubinsteinS Pestka
Jan 1, 1981·Methods in Enzymology·Y KawadeY Yamamoto
Jan 1, 1982·Journal of Interferon Research·G R BurlesonF Germain-De Matteis
Oct 1, 1984·Journal of Virological Methods·G R Burleson, D P Herzog
Jan 1, 1980·The Journal of General Virology·H SchellekensW Weimar

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