PMID: 11900113Mar 20, 2002Paper

Production and characterization of pullulan from beet molasses using a nonpigmented strain of Aureobasidium pullulans in batch culture

Applied Biochemistry and Biotechnology
Athina LazaridouMarta Izydorczyk

Abstract

The production of pullulan from beet molasses by a pigment-free strain of Aztreobasidium pullulans on shake-flask culture was investigated. Combined pretreatment of molasses with sulfuric acid and activated carbon to remove potential fermentation inhibitors present in molasses resulted in a maximum pullulan concentration of 24 g/L, a biomass dry wt of 14 g/L, a pullulan yield of 52.5%, and a sugar utilization of 92% with optimum fermentation conditions (initial sugar concentration of 50 g/L and initial pH of 7.0). The addition of other nutrients as carbon and nitrogen supplements (olive oil, ammonium sulfate, yeast extract) did not further improve the production of the exopolysaccharides. Structural characterization of the isolated polysaccharides from the fermentation broths by 13C-nuclear magnetic resonance spectroscopy and pullulanase digestion combined with size-exclusion chromatography confirmed the identity of pullulan and the homogeneity (>93% dry basis) of the elaborated polysaccharides by the microorganism. Using multiangle laser light scattering and refractive index detectors in conjunction with high-performance size-exclusion chromatography molecular size distributions and estimates of the molecular weight (Mw = 2.1-...Continue Reading

Citations

Sep 4, 2012·Bioprocess and Biosystems Engineering·Yun Lin, Jules Thibault
Apr 23, 2008·Applied Biochemistry and Biotechnology·Ram Sarup SinghGaganpreet Kaur Saini
Feb 21, 2007·Journal of Applied Microbiology·S CrognaleM Petruccioli
Jun 17, 2015·Carbohydrate Polymers·Qian XiaoFangming Deng
Nov 28, 2013·Carbohydrate Polymers·Selim SilbirYekta Goksungur
Aug 18, 2012·Journal of Biophotonics·Paolo MatteiniRoberto Pini
Aug 10, 2019·Frontiers in Genetics·Ming TanJialiang Yang
Nov 21, 2020·Applied Microbiology and Biotechnology·Sara H Al-AraimiAli S Al-Bemani
Jul 25, 2017·Carbohydrate Polymers·Sugumaran K R, Ponnusami V
Nov 5, 2020·Carbohydrate Polymers·Xin WeiZhen-Ming Chi

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