Production and characterization of the monoclonal antibodies against nonstructural 1 protein of DENV-4
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Xixia DingXiao-yan Che
To produce monoclonal antibodies (mAbs) against nonstructural 1 protein (NS1) of DENV-4 and characterization. BALB/c mice were immunized with recombinant DENV4-NS1 protein and inactive DENV-4. Splenocytes of immunized mice were fused with myeloma cells (NS-1) to produce hybridoma cell lines, secreting anti-DENV4-NS1 protein mAbs. ELISA was used to identify the mAbs against NS1of DENV-4. Immnofluorescence assay (IFA) and Western blot analysis were applied to identify the specificity of mAbs. fifteen mAbs recognizing two different antigenic epitopes on NS1of DENV-4 were obtained. These mAbs had characteristics of specific binding to recombinant NS1 of DENV-4. The IFA showed that all of the mAbs strongly reacted to DENV-4, while had no cross-reactivity to the other three serotypes of dengue virus. mAbs against NS1 of DENV-4 with high specificity has been successfully established. These results lay the foundation value for study on the structural and functional of DENV4-NS1 protein and early diagnosis of DENV-4 infection.
Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.