PMID: 21722533Jul 5, 2011Paper

Production and characterization of the monoclonal antibodies against nonstructural 1 protein of DENV-4

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Xixia DingXiao-yan Che

Abstract

To produce monoclonal antibodies (mAbs) against nonstructural 1 protein (NS1) of DENV-4 and characterization. BALB/c mice were immunized with recombinant DENV4-NS1 protein and inactive DENV-4. Splenocytes of immunized mice were fused with myeloma cells (NS-1) to produce hybridoma cell lines, secreting anti-DENV4-NS1 protein mAbs. ELISA was used to identify the mAbs against NS1of DENV-4. Immnofluorescence assay (IFA) and Western blot analysis were applied to identify the specificity of mAbs. fifteen mAbs recognizing two different antigenic epitopes on NS1of DENV-4 were obtained. These mAbs had characteristics of specific binding to recombinant NS1 of DENV-4. The IFA showed that all of the mAbs strongly reacted to DENV-4, while had no cross-reactivity to the other three serotypes of dengue virus. mAbs against NS1 of DENV-4 with high specificity has been successfully established. These results lay the foundation value for study on the structural and functional of DENV4-NS1 protein and early diagnosis of DENV-4 infection.

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