Production and characterization of exopolysaccharides by Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains isolated from an Armenian geothermal spring

Extremophiles : Life Under Extreme Conditions
Hovik PanosyanBarbara Nicolaus

Abstract

The thermal ecosystems, including geothermal springs, are proving to be source of thermophiles able to produce extracellular polysaccharides (EPSs). Among the sixteen thermophilic bacilli isolated from sediment sampled from Arzakan geothermal spring, Armenia, two best EPSs producer strains were identified based on 16S rRNA gene sequence analysis and phenotypic characteristics, and designated as Geobacillus thermodenitrificans ArzA-6 and Geobacillus toebii ArzA-8 strains. EPSs production was investigated under different time, temperature and culture media's composition. The highest specific EPSs production yield (0.27 g g-1 dry cells and 0.22 g g-1 dry cells for strains G. thermodenitrificans ArzA-6 and G. toebii ArzA-8, respectively) was observed after 24 h when fructose was used as sole carbon source at 65 °C and pH 7.0. Purified EPSs displayed a high molecular mass: 5 × 105 Da for G. thermodenitrificans ArzA-6 and 6 × 105 Da for G. toebii ArzA-8. Chemical composition and structure of the biopolymers, determined by GC-MS, HPAE-PAD and NMR, showed that both the two EPSs are heteropolymers composed by mannose as major monomer unit. Optical rotation values [α] D25 °C of the two EPSs (2 mg ml-1 H2O) were - 142,135 and - 128,645 fo...Continue Reading

References

Aug 1, 1973·Analytical Biochemistry·N Blumenkrantz, G Asboe-Hansen
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jun 8, 2000·International Journal of Systematic and Evolutionary Microbiology·P L ManachiniM G Fortina
Oct 2, 2002·Journal of Industrial Microbiology & Biotechnology·J Guezennec
Jul 30, 2005·Microbial Ecology·Carol Mancuso NicholsJean Guézennec
Sep 1, 1996·Applied and Environmental Microbiology·M C MancaB Nicolaus
Apr 19, 2007·Journal of Basic Microbiology·Anita Suresh KumarBhavanath Jha
May 13, 2011·Trends in Biotechnology·Filomena FreitasMaria A M Reis
Jun 8, 2011·Systematic and Applied Microbiology·Annarita PoliBarbara Nicolaus
Dec 6, 2011·International Journal of Systematic and Evolutionary Microbiology·Ok-Sun KimJongsik Chun
Mar 1, 2008·Indian Journal of Microbiology·Arundhati Pal, A K Paul
Dec 4, 2012·International Journal of Molecular Sciences·Uchechukwu U NwodoAnthony I Okoh
Jul 3, 2013·Applied Biochemistry and Biotechnology·Nadja RadchenkovaMargarita Kambourova
Oct 15, 2013·Journal of Applied Microbiology·Songul Yasar YildizMargarita Kambourova
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Apr 18, 2014·Journal of Basic Microbiology·Hovik Panosyan, Nils-Kåre Birkeland
Jul 20, 2014·Carbohydrate Polymers·Tereza Cristina Luque CastellaneEliana Gertrudes de Macedo Lemos
Mar 10, 2015·International Journal of Biological Macromolecules·Sreejith RaveendranD Sakthi Kumar
Jan 28, 2016·International Journal of Systematic and Evolutionary Microbiology·Ilaria FinoreAnnarita Poli
Oct 12, 2016·Systematic and Applied Microbiology·Habibu AliyuPieter De Maayer
Nov 29, 2017·Applied and Environmental Microbiology·Consolazione CarusoAngelina Lo Giudice

❮ Previous
Next ❯

Citations

Sep 11, 2019·Applied Microbiology and Biotechnology·Jia-Hui LinShuang Li
Mar 3, 2020·Frontiers in Microbiology·Pervaiz AliFeng Chen
May 12, 2020·Extremophiles : Life Under Extreme Conditions·Hovik PanosyanNils-Kåre Birkeland
Feb 21, 2021·International Journal of Biological Macromolecules·Mayra Alejandra López-OrtegaAdriana Inés Rodríguez-Hernández
Aug 8, 2021·Microorganisms·Ani SaghatelyanNils-Kåre Birkeland

❮ Previous
Next ❯

Datasets Mentioned

BETA
JQ929020

Methods Mentioned

BETA
gel filtration

Software Mentioned

BLAST
MEGA
Mallard
EzTaxon

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.