Production and Characterization of Polyhydroxyalkanoate from Lignin Derivatives by Pandoraea sp. ISTKB

ACS Omega
Madan KumarIndu Shekhar Thakur

Abstract

The present study investigates polyhydroxyalkanoate (PHA) production from lignin and its derivatives by a previously reported lignin-degrading bacterial strain Pandoraea sp. ISTKB. PHA production was screened by fluorescence microscopy and flow cytometry using a Nile red stain. PHA and biomass accumulation, while screening, was found to be maximum on 4-hydroxybenzoic acid followed by p-coumaric acid, vanillic acid, 2,6-dimethoxyphenol, and kraft lignin after 96 h. Monomer composition was analyzed by gas chromatography-mass spectrometry (GC-MS) and was followed by Fourier transform infrared and 1H NMR analysis, indicating PHA to be a copolymer of P(hydroxybutyrate-co-hydroxyvalerate). Genomic analysis of Pandoraea sp. ISTKB also complemented the results of GC-MS and NMR, and the relevant genes responsible for the synthesis of small chain length PHA were discovered in the genome. Process parameters were optimized by response surface methodology for enhanced production of PHA and biomass on 4-hydroxybenzoate. Optimization results showed 30 and 66% increase in the biomass and PHA production, respectively. The results obtained were promising and indicated that if lignin is depolymerized into low-molecular-weight intermediates, then ...Continue Reading

References

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Citations

Dec 21, 2018·Biotechnology and Applied Biochemistry·Prasun KumarBeom Soo Kim
Jul 30, 2020·Biotechnology and Applied Biochemistry·Mamata Singhvi, Beom Soo Kim
Sep 26, 2019·Frontiers in Bioengineering and Biotechnology·Siseon LeeBong Hyun Sung
Apr 23, 2020·Biotechnology for Biofuels·Chien-Yuan Lin, Aymerick Eudes
Nov 1, 2020·International Journal of Biological Macromolecules·Salma ShahidZill-I-Huma Nazli
Dec 2, 2020·Bioresource Technology·Zhaoxian XuMingjie Jin
Mar 24, 2021·Bioresource Technology·Raj MoryaIndu Shekhar Thakur
Oct 15, 2021·Critical Reviews in Biotechnology·Pavni RekhiMousumi Debnath

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Datasets Mentioned

BETA
MAOS00000000.1

Methods Mentioned

BETA
flow cytometry
nuclear magnetic resonance
NMR
confocal microscopy
FACS

Software Mentioned

Expert
Design
ISTKB

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