Production and covalent immobilisation of the recombinant bacterial carbonic anhydrase (SspCA) onto magnetic nanoparticles

Journal of Enzyme Inhibition and Medicinal Chemistry
Rosa PerfettoClemente Capasso

Abstract

Carbonic anhydrases (CAs; EC 4.2.1.1) are metalloenzymes with a pivotal potential role in the biomimetic CO2 capture process (CCP) because these biocatalysts catalyse the simple but physiologically crucial reaction of carbon dioxide hydration to bicarbonate and protons in all life kingdoms. The CAs are among the fastest known enzymes, with kcat values of up to 106 s-1 for some members of the superfamily, providing thus advantages when compared with other CCP methods, as they are specific for CO2. Thermostable CAs might be used in CCP technology because of their ability to perform catalysis in operatively hard conditions, typical of the industrial processes. Moreover, the improvement of the enzyme stability and its reuse are important for lowering the costs. These aspects can be overcome by immobilising the enzyme on a specific support. We report in this article that the recombinant thermostable SspCA (α-CA) from the thermophilic bacterium Sulfurihydrogenibium yellowstonense can been heterologously produced by a high-density fermentation of Escherichia coli cultures, and covalently immobilised onto the surface of magnetic Fe3O4 nanoparticles (MNP) via carbodiimide activation reactions. Our results demonstrate that using a bencht...Continue Reading

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Citations

Mar 15, 2018·Journal of Enzyme Inhibition and Medicinal Chemistry·Sylwia BlochGrzegorz Węgrzyn
Nov 16, 2017·Metabolites·Claudiu T Supuran, Clemente Capasso
Dec 29, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sonia Del PreteClemente Capasso
Feb 7, 2019·Journal of Enzyme Inhibition and Medicinal Chemistry·Rosa MerloGiuseppe Perugino
Aug 10, 2017·Journal of Enzyme Inhibition and Medicinal Chemistry·Sonia Del PreteClemente Capasso
Apr 27, 2021·Colloids and Surfaces. B, Biointerfaces·Sizhu RenYanlin Yuan

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Methods Mentioned

BETA
biosensors
electrophoresis
thermoprecipitation

Software Mentioned

GraphPad
GraphPad Prism

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