Production and efficacy of a low-cost recombinant pneumococcal protein polysaccharide conjugate vaccine

Vaccine
Jenny A HerbertTimothy J Mitchell

Abstract

Streptococcus pneumoniae is the leading cause of bacterial pneumonia. Although this is a vaccine preventable disease, S. pneumoniae still causes over 1 million deaths per year, mainly in children under the age of five. The biggest disease burden is in the developing world, which is mainly due to unavailability of vaccines due to their high costs. Protein polysaccharide conjugate vaccines are given routinely in the developed world to children to induce a protective antibody response against S. pneumoniae. One of these vaccines is Prevnar13, which targets 13 of the 95 known capsular types. Current vaccine production requires growth of large amounts of the 13 serotypes, and isolation of the capsular polysaccharide that is then chemically coupled to a protein, such as the diphtheria toxoid CRM197, in a multistep expensive procedure. In this study, we design, purify and produce novel recombinant pneumococcal protein polysaccharide conjugate vaccines in Escherichia coli, which act as mini factories for the low-cost production of conjugate vaccines. Recombinant vaccine efficacy was tested in a murine model of pneumococcal pneumonia; ability to protect against invasive disease was compared to that of Prevnar13. This study provides the ...Continue Reading

Citations

Apr 17, 2019·Glycobiology·Christian M Harding, Mario F Feldman
Feb 13, 2020·Biotechnology & Genetic Engineering Reviews·Thomas E MacCalmanStephen E Harding
May 10, 2019·NPJ Vaccines·Emily KayBrendan W Wren
Feb 23, 2019·Nature Communications·Christian M HardingMario F Feldman
Jul 7, 2020·Expert Review of Vaccines·Jennifer Mhairi DowBrendan William Wren
Sep 15, 2019·Molecular and Cellular Probes·Hesam DorostiYounes Ghasemi
Jun 12, 2020·ACS Synthetic Biology·Weston KightlingerMichael C Jewett
May 28, 2021·PloS One·David F ZeiglerChristopher H Clegg

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Methods Mentioned

BETA
glycosylation
protein assay
ELISA

Software Mentioned

GraphPad
Bio
Prism
Plex manager
GraphPad Prism

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