Production and evaluation of high-quality thyroxine antisera for use in radioimmunoassay

Scandinavian Journal of Clinical and Laboratory Investigation
V Kruse

Abstract

High-quality thyroxine (T4) antisera were raised in rabbits. Conjugates of protein and T4 or T4 methyl ester were given intracutaneously in Freund's complete adjuvant at one or two sites on the back at 1/2- to 3-month intervals. Eleven of 16 immunized rabbits produced antiserum with titers higher than 2 commercial antisera and 4 antisera from other laboratories. All 16 antisera had equilibrium constants (K) higher than the 6 reference sera. The highest titer observed was 130,000 with 88 fmol labeled T4 per ml incubate. A drastic increase in K from about 4 X 10(8) to 5.8 X 10(10) 1/mol was observed as the immunization proceeded. Cross-reactivity with triiodothyronine (T3) varied but was generally low. Suppression of the T4 synthesis was attempted by giving 30 nmol (20 mug) T3 per os once a day. Antiserum from rabbits given T3 suppression contained less iodine than antiserum from nonsuppressed control rabbits. The possible reasons for the successful production of the high-quality antisera are discussed.

References

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Citations

Mar 15, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·C N MpokoC C Storey
Dec 1, 1977·Scandinavian Journal of Clinical and Laboratory Investigation·P Rogowski, K Siersbaek-Nielsen
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Oct 1, 1979·Scandinavian Journal of Clinical and Laboratory Investigation·V Kruse
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Related Concepts

Binding Sites, Antibody
Cross Reactions
Immune Sera
Radioimmunoassay
Novothyral
Cytomel

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