PMID: 9630568Jun 19, 1998Paper

Production and oestrogen regulation of FGF1 in normal and cancer breast cells

Biochimica Et Biophysica Acta
I El YazidiY Boilly-Marer

Abstract

To investigate the relationship between the FGF1 oestrogen regulation and the normal/cancer status of breast cells, we have studied FGF1 17beta-oestradiol regulation in normal, transformed and cancerous cells. Normal (NMEC), transformed (HBL-100) and cancerous (MCF-7, MDA-MB-231) human mammary epithelial cells express different levels of FGF1 mRNAs. Western blot analysis allowed us to characterize FGF1 as an 18 kDa form of this polypeptide. Using a neutralizing anti-FGF1 antibody we demonstrated that FGF1 is secreted by all mammary cells studied and stimulates their proliferation in an autocrine manner. We have examined the regulation of FGF1 mRNAs in response to 17beta-oestradiol. FGF1 mRNAs were upregulated in hormone-dependent NMEC but was not upregulated either in hormone-sensitive HBL-100 cells or in the hormone-dependent cancerous cell line MCF-7. However, enzyme linked immunosorbent assay indicated an increase of FGF1 protein in NMEC, HBL-100 and MCF-7 cells. We have also examined the 17beta-oestradiol regulation of the four alternatively spliced FGF1 mRNAs: 1.A, 1.B, 1. C and 1.D. Only 1.B transcripts were downregulated by 17beta-oestradiol in normal cells. These results show that 17beta-oestradiol regulates FGF1 mRNAs ...Continue Reading

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Citations

Feb 17, 2001·The Journal of Steroid Biochemistry and Molecular Biology·E K Lobenhofer, J R Marks
May 11, 2002·Breast Cancer Research and Treatment·Rober-Alain ToillonXuefen Le Bourhis
Mar 8, 2000·Growth Factors·A Malamitsi-PuchnerG Creatsas

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