PMID: 6104990Aug 1, 1980Paper

Production and properties of antibody to soluble guanylate cyclase purified from bovine brain

Biochimica Et Biophysica Acta
M Nakane, T Deguchi

Abstract

Guanylate cyclase was purified 12,700-fold from bovine brain supernatant, and the purified enzyme exhibited essentially a single protein band on polyacrylamide gel electrophoresis. Repeated injection of the purified enzyme into rabbits produced an antibody to guanylate cyclase. The immunoglobulin G fraction from the immunized rabbit gave only one precipitin line against the purified guanylate cyclase and the crude supernatant of bovine brain on double immunodiffusion and immunoelectrophoreis. The antibody completely inhibited the soluble guanylate cyclase activity from bovine brain, various tissues of rat and mouse and neuroblastoma N1E 115 cells, whereas the Triton-dispersed particulate guanylate cyclase from these tissues was not inhibited by the antibody.

References

Sep 15, 1975·Life Sciences·H Kimura, F Murad
Jan 1, 1976·Analytical Biochemistry·A Bensadoun, D Weinstein
Jan 1, 1969·Biochimica Et Biophysica Acta·A A White, G D Aurbach
Feb 1, 1964·The Biochemical Journal·V P WhittakerR J Kirkland
Dec 28, 1964·Annals of the New York Academy of Sciences·D E WILLIAMS, R A REISFELD
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J M BraughlerF Murad

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Citations

Jan 1, 1989·Pharmacology & Therapeutics·T M Lincoln
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H BrandweinF Murad
Dec 1, 1982·Journal of Neuroimmunology·P BassetJ B Thiébaut
Apr 5, 1982·FEBS Letters·M Nakane, T Deguchi
Jun 1, 1981·European Journal of Biochemistry·R GerzerG Schultz
Dec 18, 2020·British Journal of Pharmacology·Andreas Friebe, Nils Englert

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