PMID: 20644587Jul 21, 2010Paper

Production and properties of human tumor necrosis factor peptide fragments

Bioorganicheskaia khimiia
L N ShingarovaM P Kirpichnikov

Abstract

The tumor necrosis factor (TNF) is a proinflammatory cytokine that plays a pivotal role in the regulation of the human immune system. Studies of the TNF functional topography are a challenging task in bioengineering. We have produced genes encoding the peptides Dl (3-30), D2 (31-85), D3 (86-114), and D4 (115-157), which correspond to isolated fragments of the informational structure of TNF. These genes were expressed in E. coli cells at a high level in a soluble form. We have shown that hybrid proteins SD2 and SD4 tend to form soluble aggregates, which can be destroyed by urea treatment. Purified peptides Dl, D3, and D4 possess a similar secondary structure with dominating beta-structural elements. The analysis of the biological activity of these peptides has shown that they do not exhibit cytotoxic properties on L929 murine fibroblasts. The simultaneous addition of Dl with full-length TNF results in the concentration dependent suppression of TNF activity.

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Citations

Aug 8, 2021·International Journal of Molecular Sciences·Alexei N NekrasovAnastasia A Anashkina

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