Production and properties of nanoelectrospray emitters used in Fourier transform ion cyclotron resonance mass spectrometry: implications for determination of association constants for noncovalent complexes

Analytical Chemistry
Cleidiane G ZampronioPeter J Derrick

Abstract

Electrospray ionization (ESI) is extensively used in the analysis of biological compounds; yet some fundamental properties of this technique are not completely understood. It is widely recognized that care should be exercised when noncovalent complexes are being studied by ESI, since weak noncovalent binding can be broken or formed during the desolvation process. In the present work, spectra from the noncovalent complex, vancomycin/diacetyl-L-lysyl-D-alanyl-D-alanine, obtained from ESI and from nanoelectrospray ionization (nanoESI), have been compared. The results indicated that the milder desolvation conditions arising as a result of the smaller sizes of droplets produced in the nanoESI source attenuated effects upon weak bonds in the desolvation process. The association constant values calculated from the relative peak intensities suggest that, when using ESI, the analyzed noncovalent complex dissociated in the condensed phase during the spraying process. The influences of experimental parameters such as tip diameter and coating for nanoESI needles were investigated. Principal component analysis, a multivariate analysis method, was applied to achieve a better evaluation of the spectra obtained using different needle diameters...Continue Reading

References

Oct 13, 1998·Mass Spectrometry Reviews·A G MarshallG S Jackson
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Citations

May 30, 2009·Mass Spectrometry Reviews·Graham T T GibsonRichard D Oleschuk
Dec 1, 2017·European Journal of Mass Spectrometry·Yelena YefremovaMichael O Glocker
Dec 12, 2012·European Journal of Mass Spectrometry·Lewis P MarkPeter J Derrick
Mar 6, 2019·ACS Central Science·Giang T H NguyenWilliam A Donald

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