Production and Purification of Remazol Brilliant Blue R Decolorizing Peroxidase from the Culture Filtrate of Pleurotus ostreatus.

Applied and Environmental Microbiology
K S ShinC Kim

Abstract

An extracellular H(inf2)O(inf2)-requiring Remazol brilliant blue R (RBBR) decolorizing enzymatic activity was found in the culture medium of Pleurotus ostreatus. The enzymatic activity was maximally obtained in idiophase, and the optimum C/N ratio was 24. High C/N ratios repressed the enzymatic activity, and addition of veratryl alcohol had no effect on the production of enzyme. The enzyme was purified by ammonium sulfate fractionation, Sephacryl S-200 HR chromatography, DEAE Sepharose CL-6B chromatography, and Mono Q chromatography. The purification of RBBR decolorizing peroxidase, as judged by the final specific activity of 6.00 U/mg, was 54.5-fold, with a yield of 9.9%. The molecular mass of the native enzyme determined by gel permeation chromatography was found to be about 73 kDa. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the enzyme was a monomer with a molecular mass of 71 kDa. The enzyme was optimally active at pH 3.0 to 3.5 and at 25(deg)C. Under standard assay conditions, the apparent K(infm) values of the enzyme toward RBBR and H(inf2)O(inf2) were 10.99 and 32.97 (mu)M, respectively. The enzyme had affinity toward various phenolic compounds and artificial dyes, and it was inhibited by Na(i...Continue Reading

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Citations

Dec 2, 2006·Journal of Basic Microbiology·Ivana EichlerováFrantisek Nerud
Jun 12, 2009·Critical Reviews in Biotechnology·Husain QayyumKulshrestha Yasha
Aug 8, 2013·Mycologia·Francisco J Ruiz-DueñasAngel T Martínez
Jun 8, 2011·Applied Biochemistry and Biotechnology·Chen Qi-HeSusanne Zibek
Nov 26, 2009·Folia Microbiologica·C NovotnýV Sasek
Jan 9, 2015·Environmental Science and Pollution Research International·Maria Camilla BarattoRafael Vazquez-Duhalt
Aug 12, 2016·Engineering in Life Sciences·Aslan Hwanhwi LeeByoung-Jun Ahn
Jul 12, 2012·Applied Microbiology and Biotechnology·Christoph A GasserPhilippe F-X Corvini
May 9, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·George A KrausBahram Parvin
Dec 19, 2012·World Journal of Microbiology & Biotechnology·J E Méndez-HernándezO Loera
Jul 31, 1999·Applied and Environmental Microbiology·L M LarrayaL Ramírez
Aug 2, 2001·Bioresource Technology·G M SoaresM T Pessoa de Amorim

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