Jan 1, 1976

Production and purification of the gamma haemolysin of Staphylococcus aureus 'Smith 5R'

Journal of General Microbiology
H B Fackrell, G M Wiseman

Abstract

The gamma haemolysin of Staphylococcus aureus 'Smith 5R' was produced on Dolman-Wilson agar overlain with cellophane. Maximal yields of crude lysin with titres of 2000 to 4000 haemolytic units/ml were obtained within 24 h at 37 degrees C in 10% (v/v) CO2 in air, on medium adjusted to pH 7-0. The crude lysin was purified 2700-fold (with 75% recovery) by ultrafiltration, gel filtration and ammonium sulphate fractionation. The specific activity of the lysin was 10(5) haemolytic units/mg protein after the dialysed active precipitate was extracted with NaCl and reprecipitated with ammonium sulphate. Purified gamma lysin was homogeneous by disc electrophoresis and immunoelectrophoresis.

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Mentioned in this Paper

Immunoelectrophoresis
Agar
Fractional Precipitation
Autolysin
Lysin
Gel Chromatography
Electrophoresis, Disc
Purification Aspects
Chemical Fractionation
Sodium Chloride

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