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Production and purification of the gamma haemolysin of Staphylococcus aureus 'Smith 5R'

Journal of General Microbiology

Jan 1, 1976

H B Fackrell, G M Wiseman

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Abstract

The gamma haemolysin of Staphylococcus aureus 'Smith 5R' was produced on Dolman-Wilson agar overlain with cellophane. Maximal yields of crude lysin with titres of 2000 to 4000 haemolytic units/ml were obtained within 24 h at 37 degrees C in 10% (v/v) CO2 in air, on medium adjusted to pH...read more

Mentioned in this Paper

Sodium Chloride
Fractional Precipitation
Immunoelectrophoresis
Hydrogen-Ion Concentration
Chemical Fractionation
Gel Chromatography
Purification Aspects
Molecular Sieve Chromatography
Ammonium Sulfate
Hemolysin

Production and purification of the gamma haemolysin of Staphylococcus aureus 'Smith 5R'

Journal of General Microbiology

Jan 1, 1976

H B Fackrell, G M Wiseman

PMID: 1465

DOI: 10.1099/00221287-92-1-1

Abstract

The gamma haemolysin of Staphylococcus aureus 'Smith 5R' was produced on Dolman-Wilson agar overlain with cellophane. Maximal yields of crude lysin with titres of 2000 to 4000 haemolytic units/ml were obtained within 24 h at 37 degrees C in 10% (v/v) CO2 in air, on medium adjusted to pH...read more

Mentioned in this Paper

Sodium Chloride
Fractional Precipitation
Immunoelectrophoresis
Hydrogen-Ion Concentration
Chemical Fractionation

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