Production and purification of the gamma haemolysin of Staphylococcus aureus 'Smith 5R'

Journal of General Microbiology
H B Fackrell, G M Wiseman

Abstract

The gamma haemolysin of Staphylococcus aureus 'Smith 5R' was produced on Dolman-Wilson agar overlain with cellophane. Maximal yields of crude lysin with titres of 2000 to 4000 haemolytic units/ml were obtained within 24 h at 37 degrees C in 10% (v/v) CO2 in air, on medium adjusted to pH 7-0. The crude lysin was purified 2700-fold (with 75% recovery) by ultrafiltration, gel filtration and ammonium sulphate fractionation. The specific activity of the lysin was 10(5) haemolytic units/mg protein after the dialysed active precipitate was extracted with NaCl and reprecipitated with ammonium sulphate. Purified gamma lysin was homogeneous by disc electrophoresis and immunoelectrophoresis.

Citations

Jan 1, 1982·Pharmacology & Therapeutics·J H Freer, J P Arbuthnott
May 23, 2014·Microbiology and Molecular Biology Reviews : MMBR·Francis Alonzo, Victor J Torres

Related Concepts

Ammonium Sulfate
Carbon Dioxide
Molecular Sieve Chromatography
Fractional Precipitation
Hemolysin
Hydrogen-Ion Concentration
Staphylococcus aureus

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