PMID: 11934523Apr 6, 2002Paper

Production and regulation of leptin in bovine mammary epithelial cells

Domestic Animal Endocrinology
J L Smith, L G Sheffield

Abstract

Previously, leptin has been found in human and rodent mammary tissue. The present research was conducted to determine (1) if leptin is produced by bovine mammary epithelial cells and (2) if leptin production in bovine mammary epithelial cells is hormonally regulated. Western blot analysis indicated the presence of leptin in bovine milk, while reverse transcription-polymerase chain reaction (RT-PCR) indicated the presence of leptin mRNA in mammary tissue and cultured bovine mammary epithelial cells (MAC-T cell line). A real time RT-PCR method was developed that allowed quantitative assessment of bovine leptin mRNA over approximately 3 orders of magnitude. Time course studies indicated a rapid increase in leptin mRNA in response to insulin or insulin-like growth factor-I (IGF-I). When normalized against bovine GAPDH as an internal control, 0.5 or 1h treatment with 10 ng/mL insulin gave 39+/-4 and 64+/-2-fold increase in leptin mRNA compared with 0 h control. Leptin mRNA was increased 257+/-9 and 75+/-23-fold by 0.5 or 1h treatment with 10 ng/mL IGF-I. Dose response studies indicated significant increases in leptin mRNA in response to as little as 1 ng/mL insulin or 0.1 ng/mL IGF-I. Maximum increase in leptin mRNA was observed in ...Continue Reading

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Citations

Mar 24, 2012·Animal : an International Journal of Animal Bioscience·A R G Wylie
Jan 30, 2008·BMC Genomics·Séverine A DegrelleIsabelle Hue
Jul 22, 2004·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·M MácajováM Zeman
Nov 13, 2009·Molecular Reproduction and Development·M SarkarBajram Berisha
Apr 9, 2013·Molecular and Cellular Endocrinology·Gale Newman, Ruben Rene Gonzalez-Perez
Nov 14, 2016·Journal of Dairy Science·N D SenevirathneJ A Clapper
Oct 4, 2005·Domestic Animal Endocrinology·Shinichi YonekuraYoshiaki Obara
Jan 7, 2003·Journal of Dairy Science·L F P SilvaG W Smith
Aug 9, 2003·Journal of Dairy Science·P K ChelikaniJ J Kennelly
Jul 26, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Brian J LeuryYves R Boisclair
Jul 19, 2005·Journal of Animal Science·N C WhitleyD J Jackson

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