Production and secretion of pertussis toxin subunits in Bacillus subtilis

FEMS Microbiology Letters
P SarisK Runeberg-Nyman

Abstract

Pertussis toxin (PT) is a major component of today's acellular whooping cough vaccines. The use of acellular vaccines is predicted to increase sharply in the near future. There is therefore a need to produce PT in a way that makes its purification as easy as possible. Our approach was to express all five PT subunits individually in Bacillus subtilis. We have used vectors containing the promoter and signal sequences of the alpha-amylase gene of Bacillus amyloliquefaciens followed by an insert encoding the appropriate PT-subunit. All PT-subunits were secreted and found in the culture supernatant. The level of expression varied considerably: S1 and S5 were produced in large quantities whereas much smaller amounts of S2, S3 and S4 were found. The subunits were also present in the membrane fraction of the respective strains.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·T J Gryczan, D Dubnau
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A NicosiaR Rappuoli
Dec 1, 1987·Microbial Pathogenesis·K Runeberg-NymanM Sarvas
Mar 1, 1986·Journal of General Microbiology·P KallioM Sarvas
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P LiH Inouye
Aug 1, 1985·The Journal of Pediatrics·T AoyamaT Iwata
Jun 1, 1981·Analytical Biochemistry·D S Holmes, M Quigley
May 1, 1983·Gene·I PalvaC Weissmann
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
May 16, 1981·British Medical Journal·D L MillerN S Rawson

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Citations

Oct 1, 1990·Current Opinion in Biotechnology·G Rapoport, A Klier
May 12, 2001·FEMS Microbiology Reviews·A M SmithM J Walker
Feb 5, 2005·Anais Da Academia Brasileira De Ciências·Luís C S FerreiraWolfgang Schumann
May 5, 2004·The Journal of Biological Chemistry·Inna DivinskiIllana Gozes
Mar 1, 1993·Microbiological Reviews·M Simonen, I Palva

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