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Production and specificity of antisera raised against 25-hydroxyvitamin D3-[C-3]-bovine serum albumin conjugates

Steroids

Oct 1, 1992

N KobayashiK Shimada

Abstract

In order to obtain specific antisera for use in the enzyme immunoassay of 25-hydroxyvitamin D3, three hapten-carrier conjugates having different lengths of bridges at the C-3 position were prepared from 25-hydroxyvitamin D3 by coupling with bovine serum albumin using the active ester me...read more

Mentioned in this Paper

Calcifediol, (3 beta,5E,7E)-Isomer
ALB
Immune Sera
Cross Reactions
Dose-Response Relationship, Immunologic
Chinchilla Rabbits
Spectrophotometry, Ultraviolet
Radioimmunoassay
Antibody Specificity
Serum Albumin, Bovine
1
Paper Details
References
  • References11
  • Citations5
12

Production and specificity of antisera raised against 25-hydroxyvitamin D3-[C-3]-bovine serum albumin conjugates

Steroids

Oct 1, 1992

N KobayashiK Shimada

PMID: 1455456

DOI: 10.1016/0039-128x(92)90043-9

Abstract

In order to obtain specific antisera for use in the enzyme immunoassay of 25-hydroxyvitamin D3, three hapten-carrier conjugates having different lengths of bridges at the C-3 position were prepared from 25-hydroxyvitamin D3 by coupling with bovine serum albumin using the active ester me...read more

Mentioned in this Paper

Calcifediol, (3 beta,5E,7E)-Isomer
ALB
Immune Sera
Cross Reactions
Dose-Response Relationship, Immunologic
Chinchilla Rabbits
Spectrophotometry, Ultraviolet
Radioimmunoassay
Antibody Specificity
Serum Albumin, Bovine
1

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Paper Details
References
  • References11
  • Citations5
12
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