PMID: 3756191Sep 12, 1986Paper

Production and use of an inhibitory monoclonal antibody to human lipoprotein lipase

Biochimica Et Biophysica Acta
I J GoldbergJ A Cornicelli

Abstract

Studies were performed to produce a monoclonal antibody to human lipoprotein lipase, verify the specificity of the antibody for lipoprotein lipase, and use this antibody for detection of lipoprotein lipase protein in human post-heparin plasma. Partially purified lipoprotein lipase from human milk was used as an antigen for the production of anti-lipoprotein lipase antibodies in mice. The spleen was removed from the animal having the highest titer of inhibitory antibodies to lipoprotein lipase and the cells were fused mouse myeloma cells. Culture media from the resulting hybridomas were screened for their ability to inhibit lipoprotein lipase catalytic activity. This screening procedure thus identified only those hybridomas which produced antibodies directed against lipoprotein lipase. One monoclonal antibody, from one clone, was selected for detailed study. The specificity of this antibody for lipoprotein lipase protein was established by three methods. First, post-heparin plasma lipoprotein lipase activity and immunoreactivity detected by an enzyme-linked immunosorbent assay (ELISA) co-eluted during heparin-agarose and phenyl-Sepharose chromatography. Second, the antibody detected a protein which was released into the circulat...Continue Reading

References

Jan 24, 1975·Biochimica Et Biophysica Acta·M Hamosh, P Hamosh
Feb 1, 1986·Archives of Biochemistry and Biophysics·I J GoldbergD S Goodman
Sep 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·R B GoldbergH C Seftel
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L Chao, B R Levin
Feb 1, 1982·The Journal of Clinical Investigation·A ChaitJ D Brunzell
Dec 1, 1982·The Journal of Clinical Investigation·I J GoldbergW V Brown

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Citations

Jul 16, 1993·Clinica Chimica Acta; International Journal of Clinical Chemistry·J KobayashiS Yoshida
Aug 1, 1987·Baillière's Clinical Endocrinology and Metabolism·M R Taskinen, T Kuusi
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S Ylä-HerttualaJ L Witztum
Feb 1, 1988·The Journal of Clinical Investigation·P A KernM E Pedersen
Feb 1, 1988·The Journal of Clinical Investigation·I J GoldbergF T Lindgren
Apr 1, 1987·Atherosclerosis·J EtienneJ Debray
Feb 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·G RenierD Radzioch
Jul 1, 1996·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·M AntikainenM R Taskinen
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·F CadelisP J Lupien
Jan 1, 1995·Life Sciences·A Singh-BistF B Kraemer
Oct 1, 1987·Analytical Biochemistry·J W GoersM C Schotz

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