Production, characterization, and epitope mapping of monoclonal antibodies against human polydeoxyribonucleotide kinase

Hybridoma
M FantaM Weinfeld

Abstract

Polydeoxyribonucleotide kinase (PNK) is a mammalian DNA repair enzyme that has the capacity to phosphorylate 5' DNA termini and dephosphorylate 3' DNA termini. A series of murine monoclonal antibodies (MAbs) was raised against the full-length recombinant human PNK. Seven of these antibodies were selected and characterized by enzyme immunoassay, Western blot analysis, and their capacity to immunoprecipitate PNK. The epitope location was defined by cyanogen bromide digestion and by using a truncated PNK for Western blot analysis. All of the MAbs recognize a single 60-kDa protein in human cell extracts. PNKs from calf, monkey, and Chinese hamster cell and tissue extracts were also detected by some or all of the MAbs. These antibodies can be successfully used for the cellular, biochemical, and functional analysis of PNK in different mammalian cell lines.

References

Feb 10, 1978·Biochimica Et Biophysica Acta·G E AustinG H Moyer
Oct 15, 1975·European Journal of Biochemistry·H TeraokaK Tsukada
Jun 1, 1975·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·M LennartzU Hagen
Jan 1, 1992·Cancer Investigation·A Eastman, M A Barry
Jun 18, 1985·Biochimica Et Biophysica Acta·T Bosdal, J R Lillehaug
Apr 1, 1981·European Journal of Biochemistry·S TamuraK Tsukada
Dec 31, 1982·Biochemical and Biophysical Research Communications·B H Pheiffer, S B Zimmerman
Aug 22, 1983·FEBS Letters·Y Habraken, W G Verly
Jan 1, 1994·Annual Review of Biochemistry·B Demple, L Harrison
Aug 14, 1999·The Journal of Biological Chemistry·F Karimi-BusheriM Weinfeld
May 18, 2001·Nature·J H Hoeijmakers

❮ Previous
Next ❯

Citations

Jan 3, 2012·Nucleic Acids Research·Nasser TahbazMichael Weinfeld
Aug 12, 2005·The Journal of Biological Chemistry·Sharon BarkerDavid Murray
Jan 21, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wenping ZhuRuqin Yu
Aug 23, 2020·Proceedings of the National Academy of Sciences of the United States of America·Shiladitya SenguptaSankar Mitra
May 3, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sams M A SadatAfsaneh Lavasanifar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.