Production, characterization and functional activities of v-Ski in cultured cells

Gene
B KelderJ J Kopchick

Abstract

The v-ski oncogene was introduced into mammalian cells in order to study its biochemical and biological properties. v-Ski, produced at relatively high levels by mouse L cells stably transfected with this DNA, was localized to the cell nucleus, was of correct apparent molecular mass, and was capable of complexing with DNA. Transient transfection of reporter plasmids into control or Ski producing mouse L cells revealed that Ski acts as a transcriptional activator of various transcriptional regulatory elements, including CMVie, RSV LTR and SV40. These results indicate that mouse L cells contain the nuclear cofactor(s) required for the ability of v-Ski to bind to DNA and also suggest that the v-Ski present within the cells is functional.

References

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Citations

Jul 20, 2001·American Journal of Human Biology : the Official Journal of the Human Biology Council·A Fernández-SantanderP Moral
Oct 10, 2002·Lipids·Amanda E LeonardYung-Sheng Huang
Jul 27, 2001·The International Journal of Biochemistry & Cell Biology·R A Leach, M T Tuck
Aug 28, 2001·Nucleic Acids Research·T PrathapamL Banks
May 3, 2007·Comparative Hepatology·Bruce KelderJohn J Kopchick
Mar 7, 1998·The Journal of Biological Chemistry·R Nicol, E Stavnezer

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