PMID: 9434130Jan 20, 1998Paper

Production, metabolism and effect of platelet-activating factor on the growth of the human K562 erythroid cell line

Biochimica Et Biophysica Acta
F DupuisL Michel

Abstract

The human immature K562 erythroid cell line was studied for its capacity to produce and to metabolize the phospholipid molecule platelet-activating factor (PAF). K562 cells produced PAF under calcium ionophore stimulation. Lyso PAF and acetyl-CoA (the acetate donor molecule for the acetylation of lyso PAF into PAF) had no effect on the amounts of PAF produced by ionophore-stimulated cells. The metabolism of PAF and lyso PAF by K562 cells was compared to that of freshly-isolated human bone marrow erythroblasts and blood erythrocytes. K562 cells rapidly metabolized [3H]PAF and [3H]lyso PAF with 1-alkyl analogue of phosphatidylcholine as the major metabolic product. In contrast, blood erythrocytes did not. PAF acetylhydrolase activity levels in K562 cells and bone marrow erythroblasts were similar and higher than in blood erythrocytes. PAF (1-100 nM) stimulated [3H]thymidine incorporation in K562 cells grown in low serum concentration, a non-metabolizable PAF agonist being more potent than PAF to stimulate thymidine incorporation. PAF receptor mRNA was detected in K562 cells by polymerase chain reaction on reverse transcripts. The present study demonstrates that K562 cells produce and metabolize PAF and underlines the putative rol...Continue Reading

References

Jun 1, 1992·Journal of Leukocyte Biology·M Rola-Pleszczynski, J Stankova
Jan 1, 1991·Methods in Enzymology·D M StafforiniT M McIntyre
Feb 6, 1990·Biochimica Et Biophysica Acta·J B TraversR H Fertel
Jul 1, 1995·Journal of Lipid Mediators and Cell Signalling·Y DenizotV Praloran
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E MüllerM Rola-Pleszczynski

❮ Previous
Next ❯

Citations

Nov 14, 2002·Prostaglandins & Other Lipid Mediators·Anna FallaniSalvatore Ruggieri
Sep 12, 2001·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Y DenizotB Melloni
Jan 2, 2001·Mediators of Inflammation·F TrimoreauY Denizot
Aug 17, 2006·Antioxidants & Redox Signaling·Florian LangThomas Wieder
Jun 16, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Karl S LangFlorian Lang
Aug 18, 2010·Marine Drugs·Anne-Laure DeniauAlain B Legrand
Aug 23, 2008·IUBMB Life·Michael FöllerFlorian Lang
Mar 23, 2010·Experimental Hematology·Yves Denizot
Mar 20, 2010·Carcinogenesis·Yves Denizot
Mar 29, 2000·Journal of Cellular Physiology·M BoccellinoG Camussi
Jun 1, 2006·Acta Physiologica·F LangT Wieder
Mar 30, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cristina CellaiFrancesco Paoletti
May 26, 2017·PloS One·Stefania BelvisoMiranda Menniti
Mar 3, 2005·Journal of Cell Science·Philipp A LangThomas Wieder

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.