PMID: 2125361Nov 1, 1990Paper

Production of 15-hydroxyeicosatetraenoic acid by purified human eosinophils and neutrophils

Scandinavian Journal of Immunology
E MoritaE Christophers

Abstract

In the presence of high concentrations of exogenous arachidonic acid (greater than or equal to 10 microM), eosinophils produced 15-hydroxyeicosatetraenoic acid (15-HETE) in the absence of stimuli. The calcium ionophore A23187, as well as the chemotaxins used in this study--complement split product C5a, platelet-activating factor (PAF), and N-formyl-methionyl-leucyl-phenylalanine (FMLP)--failed to increase 15-HETE production, indicating that eosinophil 15-lipoxygenase is already active. Production of 15-HETE from eosinophils increased with increasing concentrations of arachidonic acid, exogenously added. Maximal 15-HETE production was observed to be 1111 +/- 380 ng per 10(6) eosinophils at the concentration of 100 microM of arachidonic acid. With low concentrations of exogenous arachidonic acid (below 2 microM), eosinophils were considered to incorporate exogenous arachidonic acid into their cell membrane, and did not produce 15-HETE. In contrast, 15-HETE formation in highly purified neutrophils (eosinophils less than 1%) was negligible compared with that in eosinophils (300-fold less), suggesting that 15-HETE-forming activity in granulocytes is derived from the eosinophil 15-lipoxygenase pathway and that neutrophils may lack 15...Continue Reading

References

Jun 1, 1989·Scandinavian Journal of Immunology·E MoritaE Christophers
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Citations

Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R J SmithJ E Bleasdale
Jan 1, 1995·Prostaglandins, Leukotrienes, and Essential Fatty Acids·F Grimminger, P Mayser
Dec 18, 1991·European Journal of Biochemistry·T IzumiB Samuelsson
Dec 15, 1994·Biochimica Et Biophysica Acta·J T O'FlahertyM J Thomas
Jan 1, 1994·The Journal of Investigative Dermatology·N GodessartJ M de Moragas

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