Production of 3-hydroxypropionic acid in engineered Methylobacterium extorquens AM1 and its reassimilation through a reductive route

Microbial Cell Factories
Yi-Ming YangSong Yang

Abstract

3-Hydroxypropionic acid (3-HP) is an important platform chemical, serving as a precursor for a wide range of industrial applications such as the production of acrylic acid and 1,3-propanediol. Although Escherichia coli or Saccharomyces cerevisiae are the primary industrial microbes for the production of 3-HP, alternative engineered hosts have the potential to generate 3-HP from other carbon feedstocks. Methylobacterium extorquens AM1, a facultative methylotrophic α-proteobacterium, is a model system for assessing the possibility of generating 3-HP from one-carbon feedstock methanol. Here we constructed a malonyl-CoA pathway by heterologously overexpressing the mcr gene to convert methanol into 3-HP in M. extorquens AM1. The engineered strains demonstrated 3-HP production with initial titer of 6.8 mg/l in shake flask cultivation, which was further improved to 69.8 mg/l by increasing the strength of promoter and mcr gene copy number. In vivo metabolic analysis showed a significant decrease of the acetyl-CoA pool size in the strain with the highest 3-HP titer, suggesting the supply of acetyl-CoA is a potential bottleneck for further improvement. Notably, 3-HP was rapidly degraded after the transition from exponential phase to stat...Continue Reading

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Apr 14, 2020·Biotechnology Journal·Arvin Y Chen, Ethan I Lan
Jun 27, 2019·Biotechnology for Biofuels·Christian Arenas-LópezKlaus Winzer
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May 23, 2021·Journal of Applied Microbiology·L-P ZhuS Yang
Oct 5, 2019·ACS Synthetic Biology·Martina CarrilloTobias J Erb

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Datasets Mentioned

BETA
EEL86940
12932746

Methods Mentioned

BETA
enzymatic assay
PCR

Software Mentioned

SIMCA
MS excel
BLAST
MassFrontier
MS Convert
MZmine

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