Production of 3-nitrocatechol by oxygenase-containing bacteria: optimization of the nitrobenzene biotransformation by Nocardia S3

Applied Microbiology and Biotechnology
J KieboomJ A de Bont

Abstract

Twenty-one microorganisms were screened for their ability to convert nitroaromatics into 3-nitrocatechol as a result of the action of an oxygenase. Cultures containing toluene dioxygenases and phenol monooxygenases accumulated 3-nitrocatechol during incubation with nitrobenzene and nitrophenol, respectively. Nocardia S3 was selected and studied in more detail. Toluene-pregrown cultures were able to degrade nitrobenzene with a concomitant formation of 3-nitrocatechol. The rates of nitrobenzene utilization decreased throughout the biotransformation period and finally the accumulation ceased. The gradual deterioration of the biotransformation rates was not a consequence of depletion of the NADH pool, but was due to the accumulation of 3-nitrocatechol. The inhibition of nitrobenzene biotransformation by 3-nitrocatechol greatly impacts 3-nitrocatechol production processes.

Citations

Apr 9, 2011·World Journal of Microbiology & Biotechnology·Danuta WojcieszyńskaKatarzyna Hupert-Kocurek
Sep 27, 2003·Water Research·Partha Sarathi Majumder, S K Gupta
Nov 7, 2003·Population Studies·John C Caldwell, Thomas Schindlmayr

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