Production of 3R-hydroxy-polyenoic fatty acids by the yeast Dipodascopsis uninucleata

Lipids
P VenterS Nigam

Abstract

Various fatty acids were fed to the yeast Dipodascopsis uninucleata UOFS Y 128, and the extracted samples were analyzed for the accumulation of 3-hydroxy metabolites with the help of electron impact gas chromatography-mass spectrometry. Fatty acids containing of 5Z,8Z-diene system (5Z,8Z,11Z-eicosatrienoic, 5Z,8Z,11Z,14Z-eicosatetraenoic, and 5Z,8Z,11Z,14Z,17Z-eicosapentaenoic acids) yielded the corresponding 3-hydroxy-all-Z-eicosapolyenoic acids. Moreover, linoleic acid (9Z,12Z-octadecadienoic acid) and 11Z,14Z,17Z-eicosatrienoic acid were converted to the 3-hydorxylated metabolites of shorter chain length, e,g., 3-hydroxy-5Z,8Z-tetradecadienoic acid and 3-hydroxy-5Z,8Z,11Z-tetradecatrienoic acid, respectively. In contrast, no accumulation of a 3-hydroxy metabolite was observed with oleic acid (9Z-octadecenoic acid), linolelaidic acid (9E,12E-octadecadienoic acid), gamma-linolenic acid (6Z,9Z,12Z-octadecatrienoic acid), and eicosanoic acid as substrate. These findings pinpoint that the 3-hydroxylation of a fatty acid in Dipodascopsis uninucleata requires a 5Z,8Z-diene system either directly or following initial incomplete beta-oxidation. Following analysis of the enantiomer composition, the arachidonic acid metabolite was iden...Continue Reading

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Citations

May 29, 2010·Current Microbiology·Santosh NigamRupal Deva
Apr 28, 2001·FEMS Microbiology Letters·R DevaS Nigam
Oct 2, 2003·Prostaglandins & Other Lipid Mediators·Johan L F KockSantosh Nigam
Jun 12, 2013·Canadian Journal of Microbiology·Andries P S OlivierJohan Lodewyk F Kock
Jan 12, 2007·Canadian Journal of Microbiology·Desmond M NcangoJohan L F Kock
Apr 5, 2008·Canadian Journal of Microbiology·Olihile M SebolaiJohan L F Kock
Dec 24, 2009·Canadian Journal of Microbiology·Ntsoaki J LeeuwJohan L F Kock
Jul 31, 2007·Future Microbiology·John R Erb-Downward, Gary B Huffnagle
Nov 10, 2006·Antonie van Leeuwenhoek·N J LeeuwJ L F Kock
Dec 6, 2005·Antonie van Leeuwenhoek·N J LeeuwS Nigam
Jul 19, 2005·FEMS Yeast Research·Ané van HeerdenSantosh Nigam
Apr 11, 2002·Lipids·Kerstin WeilPeter Schreier
Dec 14, 2012·Mikrobiologiia·N N GesslerT A Belozerskaia
Jun 1, 2012·Expert Opinion on Drug Discovery·J Lodewyk F KockCarolina H Pohl
Nov 24, 2011·Prostaglandins & Other Lipid Mediators·Olihile M SebolaiMaurizio del Poeta
May 19, 2009·New Biotechnology·Ching T Hou
Mar 14, 2007·Pharmacology & Therapeutics·Katarzyna StarowiczVincenzo Di Marzo
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Feb 2, 2011·The FEBS Journal·Florian Brodhun, Ivo Feussner
Nov 25, 2004·Prostaglandins & Other Lipid Mediators·Olihile M SebolaiSantosh Nigam
Jul 25, 2006·International Journal of Food Microbiology·P VenterI Ivanov
Feb 3, 2007·FEMS Yeast Research·Ané van HeerdenJohan L F Kock
Jul 12, 2007·FEMS Yeast Research·Johan L F KockElizabeth J Lodolo
Mar 30, 2016·Engineering in Life Sciences·Fatma ArousTahar Mechichi
May 13, 2004·Journal of Agricultural and Food Chemistry·Sandra N E Van NieropMarina Rautenbach

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