Production of a cellulase in silkworm larvae using a recombinant Bombyx mori nucleopolyhedrovirus lacking the virus-encoded chitinase and cathepsin genes

Biotechnology Letters
Kwang Sik LeeByung Rae Jin

Abstract

The expression efficiency of an insect-derived cellulase was assayed in silkworm larvae infected with recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) mutants lacking the virus-encoded chitinase (chiA) and/or cathepsin (v-cath) genes. Expression was increased by approx. 10% in mutants lacking chiA or v-cath and 17% in a mutant lacking both chiA and v-cath compared with that of the unmodified recombinant BmNPV. The recombinant BmNPV lacking both chiA and v-cath can therefore be used for a large-scale production of foreign proteins in silkworms.

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Citations

Oct 16, 2009·Applied Microbiology and Biotechnology·Tatsuya KatoEnoch Y Park
Nov 9, 2006·Biotechnology Letters·Kwang Sik LeeByung Rae Jin
Jun 1, 2011·Applied Biochemistry and Biotechnology·Xing-Hua LiYun-Gen Miao
Jun 8, 2013·Protein Expression and Purification·Fuxiao LiuZhiliang Wang
May 1, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Young Joo KimByung Rae Jin
Sep 29, 2011·Methods : a Companion to Methods in Enzymology·Richard B HitchmanLinda A King

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